製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to UBQLN4
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to 18 aa from near the N-terminus of Human UBQLN4 (Q9NRR5).

  • ポジティブ・コントロール
    • 3T3 cell lysate, Mouse kidney tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (stable for up to 12 months).
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 64 kDa.

ターゲット情報

  • 組織特異性
    Highly expressed in pancreas, kidney, skeletal muscle, heart and throughout the brain, and at lower levels in placenta, lung and liver.
  • 配列類似性
    Contains 1 UBA domain.
    Contains 1 ubiquitin-like domain.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus. Cytoplasm. Endoplasmic reticulum. May associate with the endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 28S ribosomal protein S7 antibody
    • A1U antibody
    • A1Up antibody
    • AI663987 antibody
    • Ataxin 1 interacting ubiquitin like protein antibody
    • Ataxin 1 ubiquitin like interacting protein A1U antibody
    • Ataxin 1 ubiquitin like interacting protein antibody
    • Ataxin-1 ubiquitin-like-interacting protein A1U antibody
    • bMRP 27a antibody
    • bMRP27a antibody
    • C1orf6 antibody
    • CIP75 antibody
    • Connexin43 interacting protein of 75 kDa antibody
    • MRP S7 antibody
    • RGD1308273 antibody
    • S7mt antibody
    • UBIN antibody
    • Ubiquilin 4 antibody
    • Ubiquilin-4 antibody
    • UBQL4_HUMAN antibody
    • UBQLN4 antibody
    see all

Anti-UBQLN4 antibody 画像

  • Lane 1 : Anti-UBQLN4 antibody (ab106443) at 1 µg/ml
    Lane 2 : Anti-UBQLN4 antibody (ab106443) at 2 µg/ml

    Lane 1 : 3T3 cell lysate
    Lane 2 : 3T3 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 64 kDa
  • ab106443, at 5 ug/ml, staining UBQLN4 in Mouse kidney tissue by immunohistochemistry.

Anti-UBQLN4 antibody (ab106443) 使用論文

This product has been referenced in:
  • Itakura E  et al. Ubiquilins Chaperone and Triage Mitochondrial Membrane Proteins for Degradation. Mol Cell 63:21-33 (2016). WB ; Human . Read more (PubMed: 27345149) »
  • Coffey RT  et al. Ubiquilin-mediated Small Molecule Inhibition of Mammalian Target of Rapamycin Complex 1 (mTORC1) Signaling. J Biol Chem 291:5221-33 (2016). Read more (PubMed: 26740621) »

See all 2 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Rat Tissue sections (forebrain)
Specification
forebrain
Permeabilization
Yes - triton-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 02 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Rat Tissue lysate - whole (HEART)
Specification
HEART
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jan 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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