製品の概要

  • 製品名Anti-UBPY/USP8 antibody
    UBPY/USP8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to UBPY/USP8
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH conjugated synthetic peptide (10-30 aa in length) at the N-term of first 50 aa of human USP8.

  • ポジティブ・コントロール
    • Transfected 293T cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).

ターゲット情報

  • 機能Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1.
  • 配列類似性Belongs to the peptidase C19 family.
    Contains 1 MIT domain.
    Contains 1 rhodanese domain.
    Contains 1 USP domain.
  • ドメインThe MIT domain is required for endosomal localization, CHMP1B-binding, maintenance of ESCRT-0 stability and EGFR degradation.
    The rhodanese domain is sufficient for RNF41-binding.
  • 翻訳後修飾Phosphorylation of Ser-718 is essential for interaction with YWHAE and for cytosol localization. Undergoes dephosphorylation at Ser-718 in the M phase. Tyrosine-phosphorylated in its N-terminal half in an EGFR-dependent manner.
    Ubiquitinated. Inactive form is mostly monoubiquitinated, but polyubiquitination happens too. Ubiquitination is increased in EGF-stimulated cells. Ubiquitination of active form is undetectable, suggesting a possibility that USP8 deubiquitinates itself, thereby regulating its own function.
  • 細胞内局在Cytoplasm. Nucleus. Endosome membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Deubiquitinating enzyme 8 antibody
    • FLJ34456 antibody
    • hUBPy antibody
    • HumORF8 antibody
    • KIAA0055 antibody
    • MGC129718 antibody
    • Ubiquitin carboxyl-terminal hydrolase 8 antibody
    • Ubiquitin isopeptidase Y antibody
    • ubiquitin specific peptidase 8 antibody
    • Ubiquitin thioesterase 8 antibody
    • Ubiquitin-specific-processing protease 8 antibody
    • UBP8_HUMAN antibody
    • UBPY antibody
    • Usp8 antibody
    see all

Anti-UBPY/USP8 antibody 画像

  • Anti-UBPY/USP8 antibody (ab59949) at 1/100 dilution + Transfected 293T cells

    Predicted band size : 128 kDa
    Observed band size : 128 kDa

Anti-UBPY/USP8 antibody (ab59949) 使用論文

This product has been referenced in:
  • Panner A  et al. Ubiquitin-specific protease 8 links the PTEN-Akt-AIP4 pathway to the control of FLIPS stability and TRAIL sensitivity in glioblastoma multiforme. Cancer Res 70:5046-53 (2010). WB ; Human, Mouse . Read more (PubMed: 20484045) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS
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Dr. Kirk McManus

Verified customer

投稿 Sep 07 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Platelets)
Specification Platelets
Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy Platelets
Username

Abcam user community

Verified customer

投稿 Dec 21 2010

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (PMN)
Specification PMN
Fixative Formaldehyde
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 2%
Username

Abcam user community

Verified customer

投稿 Feb 18 2010

Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 5 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (12.5 % gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Oct 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"