製品の概要

  • 製品名Anti-UBPY/USP8 antibody
    UBPY/USP8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to UBPY/USP8
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH selected within aa 1050~1100 of human USP8.

  • ポジティブ・コントロール
    • Human breast carcinoma and hepatocarcinoma tissues and transfected 293T cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation. Converts both 'Lys-48' an 'Lys-63'-linked ubiquitin chains. Catalytic activity is enhanced in the M phase. Involved in cell proliferation. Required to enter into S phase in response to serum stimulation. May regulate T-cell anergy mediated by RNF128 via the formation of a complex containing RNF128 and OTUB1. Probably regulates the stability of STAM2 and RASGRF1. Regulates endosomal ubiquitin dynamics, cargo sorting, membrane traffic at early endosomes, and maintenance of ESCRT-0 stability. The level of protein ubiquitination on endosomes is essential for maintaining the morphology of the organelle. Deubiquitinates EPS15 and controles tyrosine kinase stability. Removes conjugated ubiquitin from EGFR thus regulating EGFR degradation and downstream MAPK signaling. Involved in acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules. Deubiquitinates BIRC6/bruce and KIF23/MKLP1.
  • 配列類似性Belongs to the peptidase C19 family.
    Contains 1 MIT domain.
    Contains 1 rhodanese domain.
    Contains 1 USP domain.
  • ドメインThe MIT domain is required for endosomal localization, CHMP1B-binding, maintenance of ESCRT-0 stability and EGFR degradation.
    The rhodanese domain is sufficient for RNF41-binding.
  • 翻訳後修飾Phosphorylation of Ser-718 is essential for interaction with YWHAE and for cytosol localization. Undergoes dephosphorylation at Ser-718 in the M phase. Tyrosine-phosphorylated in its N-terminal half in an EGFR-dependent manner.
    Ubiquitinated. Inactive form is mostly monoubiquitinated, but polyubiquitination happens too. Ubiquitination is increased in EGF-stimulated cells. Ubiquitination of active form is undetectable, suggesting a possibility that USP8 deubiquitinates itself, thereby regulating its own function.
  • 細胞内局在Cytoplasm. Nucleus. Endosome membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Deubiquitinating enzyme 8 antibody
    • FLJ34456 antibody
    • hUBPy antibody
    • HumORF8 antibody
    • KIAA0055 antibody
    • MGC129718 antibody
    • Ubiquitin carboxyl-terminal hydrolase 8 antibody
    • Ubiquitin isopeptidase Y antibody
    • ubiquitin specific peptidase 8 antibody
    • Ubiquitin thioesterase 8 antibody
    • Ubiquitin-specific-processing protease 8 antibody
    • UBP8_HUMAN antibody
    • UBPY antibody
    • Usp8 antibody
    see all

Anti-UBPY/USP8 antibody 画像

  • Anti-UBPY/USP8 antibody (ab38865) + WiDr cell lysate

    Predicted band size : 128 kDa
  • Formalin fixed and paraffin embedded human breast carcinoma tissue stained with ab38865, followed by AEC staining.

Anti-UBPY/USP8 antibody (ab38865) 使用論文

ab38865 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your message and for providing this further information. Reviewing the details, there are regrettably no major suggestions to provide to help improve the results on this occasion. I appreciate the time you have spent on these experiments ...

Read More

Thank you for your telephone call this morning. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. We will happily offer technical support and in the event that a product is not functioning in the applicat...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"