製品の概要

  • 製品名Anti-Ubp64E antibody
    Ubp64E 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to Ubp64E
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Drosophila melanogaster
  • 免疫原

    Synthetic peptide:

    LPDLYKPRVYFYNPSLKKMK

    , corresponding to amino acids 485-504 of Fruit fly (Drosophila melanogaster) Ubp64E

  • 特記事項

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 32.2 kDa (predicted molecular weight: 174 kDa).

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 関連性Ubp64E is a ubiquitin specific protease, and has an effect on position effect variegation. It belongs to the peptidase C19 family.
  • 細胞内局在Nuclear
  • 参照データベース
    • 別名
      • Deubiquitinating enzyme 64E antibody
      • Ubiquitin specific protease 64E antibody
      • Ubiquitin thiolesterase 64E antibody
      • UBP antibody
      • Ubp64 antibody
      see all

    Anti-Ubp64E antibody 画像

    • All lanes : Anti-Ubp64E antibody (ab52688) at 1/1000 dilution

      Lane 1 : The negative control lane: ~20ug of a total protein extract from E coli with ~50ng to 100 ng of a recombinant fusion protein of an irrelevant antigen.
      Lane 2 : Test lane: ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein)

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 174 kDa
      Observed band size : 32.2 kDa (why is the actual band size different from the predicted?)

    Anti-Ubp64E antibody (ab52688) 使用論文

    ab52688 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"