製品の概要

  • 製品名Anti-UBN1 antibody [UBN1-02]
    UBN1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [UBN1-02] to UBN1
  • アプリケーション適用あり: Flow Cyt, WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to N terminal amino acids 1-190 of Human UBN1

  • エピトープN terminal.
  • ポジティブ・コントロール
    • HeLa nuclear cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 165 kDa (predicted molecular weight: 122 kDa).
IP Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 関連性UBN1 may be required for replication independent chromatin assembly, and interacts with epstein-barr virus BZLF1 and CEBPA. It is ubiquitous, and is also expressed in numerous tumours and cancer cell lines. Interacts with epstein-barr virus BZLF1 and CEBPA.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • Ubinuclein antibody
    • Ubiquitously expressed nuclear protein antibody
    • VT4 antibody

Anti-UBN1 antibody [UBN1-02] 画像

  • Overlay histogram showing HeLa cells stained with ab84953 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84953, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG ( 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Lane 1 : Anti-UBN1 antibody [UBN1-02] (ab84953) at 1 µg/ml
    Lane 2 : Anti-UBN1 antibody [UBN1-02] (ab84953) at 5 µg/ml

    Lane 1 : HeLa nuclear lysate
    Lane 2 : HeLa nuclear lysate


    Predicted band size : 122 kDa
    Observed band size : 165 kDa (why is the actual band size different from the predicted?)

Anti-UBN1 antibody [UBN1-02] (ab84953) 使用論文

ab84953 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (K562 (human erythroid cell line))
Negative control normal IgG
Specification K562 (human erythroid cell line)
Detection step Taqman
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Positive control H2A.Z antibody
Username

Abcam user community

Verified customer

投稿 Oct 10 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"