The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).
Dephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity. The dephosphorylation may prevent assembly of the core and regulatory particles (CP and RP) into mature 26S proteasome.
Broadly expressed, with highest levels in placenta, lung, testis and ovary. Up-regulated in tumor tissues.