製品の概要

  • 製品名Anti-Ubiquitin antibody
    Ubiquitin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ubiquitin
  • 特異性It can identify free ubiquitin as well as ubiquinated proteins. The antibody recognizes polyubiquitin chains more strongly than monoubiquitinated molecules.
  • アプリケーション適用あり: Immunoelectrophoresis, IHC-FoFr, ICC/IF, WB, IPmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Rat, Sheep, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Fish, Monkey, Escherichia coli
  • 免疫原

    Full length native protein (purified) corresponding to Cow Ubiquitin conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • Heat-shocked HeLa cell lysate, mouse brain tissue extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Immunoelectrophoresis 1/1000.
IHC-FoFr 1/200.
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Predicted molecular weight: 10 kDa.
IP 1/100. PubMed: 20103532
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 関連性Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
    • 細胞内局在Cell Membrane, Cytoplasmic and Nuclear
    • 参照データベース
    • 別名
      • FLJ25987 antibody
      • MGC8385 antibody
      • Polyubiquitin B antibody
      • RPS 27A antibody
      • RPS27A antibody
      • UBA 52 antibody
      • UBA 80 antibody
      • UBA52 antibody
      • UBA80 antibody
      • UBB antibody
      • UBB_HUMAN antibody
      • UBC antibody
      • UBCEP 1 antibody
      • UBCEP 2 antibody
      • UBCEP1 antibody
      • UBCEP2 antibody
      • Ubiquitin antibody
      • Ubiquitin B antibody
      see all

    Anti-Ubiquitin antibody 画像

    • Lane 1 :
      Lane 2 :
      Lane 3 :

      Lane 1 : MCF-7
      Lane 2 : MCF-7 + (50 uM 90 min)
      Lane 3 : Mouse Brain

      Lysates/proteins at 20 µg per lane.


      Performed under reducing conditions.

      Predicted band size : 10 kDa

      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes.  ab19247 and ab8245 (loading control to GAPDH) were diluted 1/200  and 1/10 000 respectively and incubated overnight at 4°C.  Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging using the Licor Odyssey CLx.

    • Anti-Ubiquitin antibody (ab19247) at 1/1000 dilution + Heat-shocked HeLa cell lysate

      Predicted band size : 10 kDa
    • Scrambled and lentivirus-stable infected SN4741 cells grown on coverslips were induced to differentiate under restrictive conditions for 24 h (pre-lethal stage), fixed and permeabilized with 0.1% Triton X-100 + 0.1% sodium citrate for 5 minutes. Cells were blocked with 10% donkey serum in PBS for 1 hour at 37°C and incubated over night at 4°C with rabbit polyclonal antibody against ubiquitin (ab19247, 1:100) and mouse monoclonal α-synuclein antibody (1:25). Samples were washed twice with PBS for 10 minutes before the addition of secondary antibodies at room temperature in the dark for 1 hour

      Scrambled infected (control) cells show a diffuse staining pattern and do not show any inclusions at both permissive and restrictive temperatures (upper images). SKP1A-deficient cells developed multiple and perinuclear (bottom images, see arrows and insets) inclusion bodies 24 h after induction of differentiation, with characteristics of aggresomes. The reactivity of the aggregates to the different antibodies demonstrated a similar pattern as indicated by co-localized yellow immunostaining in the overlaid right-hand panel. No fluorescence was detected when the primary antibody was omitted. Each panel shows a representative picture of 10–15 views in two independent experiments.

      Images top - bottom:

      Scrambled 33°C/10% FCS,

      SKP1A shRNA-1 33°C/10% FCS,

      Scrambled 39°C/0.5% FCS, 

      SKP1A shRNA-1 39°C/0.5% FCS.

    Anti-Ubiquitin antibody (ab19247) 使用論文

    This product has been referenced in:
    • Chen X & Chalfie M Regulation of mechanosensation in C. elegans through ubiquitination of the MEC-4 mechanotransduction channel. J Neurosci 35:2200-12 (2015). Read more (PubMed: 25653375) »
    • Yang F  et al. BubR1 Is Modified by Sumoylation during Mitotic Progression. J Biol Chem 287:4875-82 (2012). WB ; Human . Read more (PubMed: 22167194) »

    See all 8 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (EC CELLS)
    Gel Running Conditions Non-reduced Non-Denaturing (Native)
    Loading amount 60 µg
    Specification EC CELLS
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 08 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Mouse mammary cell)
    Loading amount 250 µg
    Specification Mouse mammary cell
    Treatment 5ng/ml TGF beta for 8hrs
    Gel Running Conditions Reduced Denaturing (10.0% gel)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    投稿 Sep 07 2011

    Thank you for your enquiry. Further to correspondence with the source of this antibody I can tell you that the following approach was employed for the testing of this antibody by western blot analysis. In brief the samples were prepared in denaturi...

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    Application Western blot
    Sample African Green Monkey Cell lysate - whole cell (Vero)
    Loading amount 30000 cells
    Specification Vero
    Treatment CO-IP for the prot of interest (40kDa)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
    Username

    Mr. olivier Reynard

    Verified customer

    投稿 Jul 27 2006

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Saccharomyces cerevisiae Cell lysate - whole cell (Saccharomyces cerevisiae)
    Total protein in input 5 µg
    Specification Saccharomyces cerevisiae
    Immuno-precipitation step Protein G
    Username

    Abcam user community

    Verified customer

    投稿 Jun 19 2006

    Thank you for your enquiry. It has not been determined whether ab14372 detects mono- or poly-ubiquitinated proteins. It would quite likely recognize both. Ab19247 should recognize ubiquinated proteins in Western blots regardless of how may ubiq...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"