製品の概要

  • 製品名Anti-Ubiquitin antibody [1B4-UB]
    Ubiquitin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1B4-UB] to Ubiquitin
  • アプリケーション適用あり: IHC-P, IHC-Fr, WB, IHC-FoFr, ELISA, Flow Cytmore details
  • 種交差性
    交差種: Cow, Human
  • 免疫原

    Ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin

  • ポジティブ・コントロール
    • Human hypophysis

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/1000.
IHC-Fr 1/500 - 1/1000.
WB Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt 1/10 - 1/50.

ターゲット情報

  • 関連性Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • 細胞内局在Cell Membrane, Cytoplasmic and Nuclear
  • 参照データベース
  • 別名
    • FLJ25987 antibody
    • MGC8385 antibody
    • Polyubiquitin B antibody
    • RPS 27A antibody
    • RPS27A antibody
    • UBA 52 antibody
    • UBA 80 antibody
    • UBA52 antibody
    • UBA80 antibody
    • UBB antibody
    • UBB_HUMAN antibody
    • UBC antibody
    • UBCEP 1 antibody
    • UBCEP 2 antibody
    • UBCEP1 antibody
    • UBCEP2 antibody
    • Ubiquitin antibody
    • Ubiquitin B antibody
    see all

Anti-Ubiquitin antibody [1B4-UB] 画像

  • ab122 staining Ubiquitin in the tissue of human hypophysis by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-Ubiquitin antibody [1B4-UB] (ab122) 使用論文

This product has been referenced in:
  • Shevade S  et al. Food vacuole associated enolase in plasmodium undergoes multiple post-translational modifications: evidence for atypical ubiquitination. PLoS One 8:e72687 (2013). Read more (PubMed: 24009698) »
  • Gao Z  et al. Sirtuin 1 (SIRT1) Protein Degradation in Response to Persistent c-Jun N-terminal Kinase 1 (JNK1) Activation Contributes to Hepatic Steatosis in Obesity. J Biol Chem 286:22227-34 (2011). WB ; Human . Read more (PubMed: 21540183) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Breast cancer Cells)
Loading amount 50 µg
Specification Breast cancer Cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 12 2008

This is a relatively new antibody that has not yet been epitope mapped or tried in yeast. We are about to implement a trial 'try risk free' scheme which says that if an antibody is purchased and used in an experiment outside that written on the datashe...

Read More

A single band at 32kD is detected in Western blotting. Although we do have pictures of Western blots, these are of very poor quality and therefore of little help.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"