Anti-Ubiquitin +1 抗体 [40B3] (ab24259)

製品の概要

  • 製品名Anti-Ubiquitin +1 antibody [40B3]
  • 製品の詳細
    Mouse monoclonal [40B3] to Ubiquitin +1
  • アプリケーション適用あり: ELISA, IP, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (Human) conjugated to KLH

  • ポジティブ・コントロール
    • Bosc23 cell lysate transfected with GFP Ub +1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab24259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
IP
WB
  • 追加情報ELISA: Use at an assay dependent dilution.
    IP: Use at a concentration of 1 - 2 µg/ml cell lysate.
    WB: 1/500 - 1/1000. Predicted molecular weight: 9 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Ubiquitin +1 (Ub+1) is a novel mutant form of ubiquitin that can be produced through a process known as molecular frameshift. Ub+1 can be polyubiquitinated to produce aberrant polyubiquitin chains that inhibit the 26S proteasome. Especially Ub+1 is accumulated in aggregates containing amyloid beta and phosphorylated tau. Elevated expression of Ub+1 mRNA and protein has been observed in the brains of patients with Alzheimer's disease. Also Ub+1 acts as an aggravating factor in polyglutamine induced neurodegeneration.
    • 細胞内局在Cytoplasmic and Nuclear
    • 参照データベース
    • 別名
      • UBB antibody
      • Ubiquitin antibody
      • Ubiquitin B antibody

    Anti-Ubiquitin +1 antibody [40B3] (ab24259) 使用論文

    ab24259 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Human Tissue lysate - other (Human Patient Blood (EDTA preserved))
    Gel Running Conditions Reduced Denaturing (4-20% Bis-TRIS)
    Loading amount 1 µg
    Treatment TCA Precipitated
    Specification Human Patient Blood (EDTA preserved)
    Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Dr. Mark Nakasone

    Verified customer

    投稿 Dec 01 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"