The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ELISA: Use at an assay dependent dilution.
IP: Use at a concentration of 1 - 2 µg/ml cell lysate.
WB: 1/500 - 1/1000. Predicted molecular weight: 9 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
関連性Ubiquitin +1 (Ub+1) is a novel mutant form of ubiquitin that can be produced through a process known as molecular frameshift. Ub+1 can be polyubiquitinated to produce aberrant polyubiquitin chains that inhibit the 26S proteasome. Especially Ub+1 is accumulated in aggregates containing amyloid beta and phosphorylated tau. Elevated expression of Ub+1 mRNA and protein has been observed in the brains of patients with Alzheimer's disease. Also Ub+1 acts as an aggravating factor in polyglutamine induced neurodegeneration.