Anti-UBF1 (phospho S484) 抗体 [EPR2725(2)] (ab182583)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2725(2)] to UBF1 (phospho S484)
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-UBF1 (phospho S484) antibody [EPR2725(2)]
UBF1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2725(2)] to UBF1 (phospho S484) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Nocodazole treated HeLa cell lysate; Human colon and squamous cell carcinoma of cervix tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2725(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab182583の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 94-97 kDa (predicted molecular weight: 89 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 94-97 kDa (predicted molecular weight: 89 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. -
配列類似性
Contains 6 HMG box DNA-binding domains. -
翻訳後修飾
Phosphorylated and activated by PIK3CA. -
細胞内局在
Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 7343 Human
- Entrez Gene: 21429 Mouse
- Entrez Gene: 25574 Rat
- Omim: 600673 Human
- SwissProt: P17480 Human
- SwissProt: P25976 Mouse
- SwissProt: P25977 Rat
- Unigene: 89781 Human
see all -
別名
- 90 kDa nucleolus organizer region autoantigen antibody
- Autoantigen NOR-90 antibody
- NOR 90 antibody
see all
画像
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All lanes : Anti-UBF1 (phospho S484) antibody [EPR2725(2)] (ab182583) at 1/10000 dilution
Lane 1 : Nocodazole treated HeLa cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 89 kDa -
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UBF1 with ab182583 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling UBF1 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (6)
ab182583 は 6 報の論文で使用されています。
- Lawrence MM et al. Muscle from aged rats is resistant to mechanotherapy during atrophy and reloading. Geroscience 43:65-83 (2021). PubMed: 32588343
- Xu X et al. Therapeutic efficacy of the novel selective RNA polymerase I inhibitor CX-5461 on pulmonary arterial hypertension and associated vascular remodelling. Br J Pharmacol 178:1605-1619 (2021). PubMed: 33486761
- Lawrence MM et al. Massage as a mechanotherapy promotes skeletal muscle protein and ribosomal turnover but does not mitigate muscle atrophy during disuse in adult rats. Acta Physiol (Oxf) 229:e13460 (2020). PubMed: 32125770
- Kotani T et al. Consecutive bouts of electrical stimulation-induced contractions alter ribosome biogenesis in rat skeletal muscle. J Appl Physiol (1985) 126:1673-1680 (2019). PubMed: 30998122
- Ye Q et al. Therapeutic Targeting of RNA Polymerase I With the Small-Molecule CX-5461 for Prevention of Arterial Injury-Induced Neointimal Hyperplasia. Arterioscler Thromb Vasc Biol 37:476-484 (2017). PubMed: 28062495
- Crossland H et al. A dynamic ribosomal biogenesis response is not required for IGF-1-mediated hypertrophy of human primary myotubes. FASEB J 31:5196-5207 (2017). PubMed: 28774889