製品の概要

  • 製品名Anti-UBE4B antibody
    UBE4B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to UBE4B
  • アプリケーション適用あり: IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1252 and 1302 of human UBE4B (AAD02233.1)

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab72501 was affinity purified using an epitope specific to UBE4B immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2-5 µg/mg of lysate.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
    • 組織特異性Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
    • 配列類似性Belongs to the ubiquitin conjugation factor E4 family.
      Contains 1 U-box domain.
    • 翻訳後修飾Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 4930551I19Rik antibody
      • 4933406G05Rik antibody
      • AU014668 antibody
      • D4Bwg0973e antibody
      • E4 antibody
      • HDNB1 antibody
      • Homozygously deleted in neuroblastoma 1 antibody
      • KIAA0684 antibody
      • mKIAA0684 antibody
      • RP23 173D8.1 antibody
      • Ube4b antibody
      • UBE4B_HUMAN antibody
      • Ubiquitin conjugation factor E4 B antibody
      • Ubiquitin fusion degradation protein 2 antibody
      • UBOX3 antibody
      • UFD2 antibody
      • UFD2a antibody
      • Ufd2p antibody
      see all

    Anti-UBE4B antibody 画像

    • Detection of UBE4B by Western Blot of Immunprecipitate.
      ab72497 at 0.1µg/ml staining UBE4B in HeLa whole cell lysate immunoprecipitated using ab72501 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 minutes.

    Anti-UBE4B antibody (ab72501) 使用論文

    ab72501 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (U2OS)
    Specification U2OS
    Fixative Paraformaldehyde
    Permeabilization Yes - triton 0.5%
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    投稿 Dec 02 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"