製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to UBE3C
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length UBE3C protein (Human)

  • ポジティブ・コントロール
    • UBE3C transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 124 kDa).

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.
  • 組織特異性
    Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 IQ domain.
  • ドメイン
    The C-terminal is necessary and sufficient for the poly-ubiquitin chain assembly.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HectH2 antibody
    • KIAA0010 antibody
    • KIAA10 antibody
    • Ube3c antibody
    • UBE3C_HUMAN antibody
    • Ubiquitin protein isopeptide ligase (E3) antibody
    • Ubiquitin protein ligase E3C antibody
    • Ubiquitin-protein ligase E3C antibody
    see all

画像

  • All lanes : Anti-UBE3C antibody (ab68225) at 1/500 dilution

    Lane 1 : UBE3C transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 124 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab68225 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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