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Our Abpromise guarantee covers the use of ab3519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||1/2000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).
By Western blot, this antibody detects a single ~100 kDa protein representing E6-AP from HeLa cell lysate. IMPORTANT: E6-AP is a very labile protein. Freeze/thaw cycling of samples results in E6-AP degradation which is observed as a prominent 50 kDa band seen in Western blots.
|IHC-Fr||Use at an assay dependent concentration.|
Western blot detection of UBE3A in HeLa cell extract using ab3519.
ab3519 has not yet been referenced specifically in any publications.