製品の概要

  • 製品名Anti-UBE3A antibody
    UBE3A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to UBE3A
  • アプリケーション適用あり: IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human ubiquitin protein ligase E3A encoded within exon 6 (LocusLink ID 7337).

  • ポジティブ・コントロール
    • Cytoplasmic fraction from HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab10487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
  • 追加情報IP: Use at a concentration of 1 - 4 µg/mg of cell lysate.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).
    • パスウェイProtein modification; protein ubiquitination.
    • 関連疾患Angelman syndrome
    • 配列類似性Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    • 翻訳後修飾Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells.
    • 細胞内局在Nucleus. Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ANCR antibody
      • Angelman syndrome antibody
      • AS antibody
      • CTCL tumor antigen se37 2 antibody
      • CTCL tumor antigen se37-2 antibody
      • E6 AP antibody
      • E6AP antibody
      • E6AP ubiquitin protein ligase antibody
      • E6AP ubiquitin-protein ligase antibody
      • EPVE6AP antibody
      • FLJ26981 antibody
      • HECT-type ubiquitin transferase E3A antibody
      • HPVE6A antibody
      • Human papilloma virus E6 associated protein Angelman syndrome antibody
      • Human papilloma virus E6 associated protein antibody
      • Human papillomavirus E6-associated protein antibody
      • NY REN 54 antigen antibody
      • Oncogenic protein associated protein E6 AP antibody
      • Oncogenic protein associated protein E6AP antibody
      • Oncogenic protein-associated protein E6-AP antibody
      • Renal carcinoma antigen NY REN 54 antibody
      • Renal carcinoma antigen NY-REN-54 antibody
      • UBE 3A antibody
      • Ube3a antibody
      • UBE3A protein antibody
      • UBE3A_HUMAN antibody
      • Ubiquitin protein ligase E3A antibody
      • Ubiquitin-protein ligase E3A antibody
      see all

    Anti-UBE3A antibody 画像

    • ab10487 immunoprecipitation of  UBE3A (E6AP). Samples: Cytoplasmic (S100) fraction (10 mg) from HeLa cells. Antibody: ab10487 used at 20 µg/10 mg extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is UBE3A (E6AP).


    Anti-UBE3A antibody (ab10487) 使用論文

    ab10487 has not yet been referenced specifically in any publications.

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