製品の概要

  • 製品名
    Anti-UBE2S antibody [AT2C12]
    UBE2S 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AT2C12] to UBE2S
  • アプリケーション
    適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human E2 EPF protein (NP_055316.2) purified from E. coli.

  • ポジティブ・コントロール
    • WB: 293T cell lysates ICC/IF: HeLa cells
  • 特記事項


    ab93904 is derived from hybridization of Mouse F0 myeloma cells with spleen cells from BALB/c mice.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250 - 1/500. Predicted molecular weight: 24 kDa.
ELISA 1/250 - 1/500.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Belongs to the ubiquitin-conjugating enzyme family.
  • 翻訳後修飾
    Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AA409170 antibody
    • E2 EPF antibody
    • E2-EPF antibody
    • E2EPF antibody
    • EPF5 antibody
    • MGC101982 antibody
    • Ube2s antibody
    • UBE2S_HUMAN antibody
    • Ubiquitin carrier protein S antibody
    • Ubiquitin conjugating enzyme E2 24 kD antibody
    • Ubiquitin conjugating enzyme E2 24 kDa antibody
    • Ubiquitin conjugating enzyme E2 EPF5 antibody
    • Ubiquitin conjugating enzyme E2 S antibody
    • Ubiquitin conjugating enzyme E2S antibody
    • Ubiquitin protein ligase S antibody
    • Ubiquitin-conjugating enzyme E2 S antibody
    • Ubiquitin-conjugating enzyme E2-24 kDa antibody
    • Ubiquitin-conjugating enzyme E2-EPF5 antibody
    • Ubiquitin-protein ligase S antibody
    see all

画像

  • Anti-UBE2S antibody [AT2C12] (ab93904) at 1/250 dilution + 293T cell lysates at 50 µg

    Secondary
    Goat anti Mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 24 kDa
  • Immunofluorescence analysis of Human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining, and ab93904 (1/500 dilution) with Alexa 488 (Green).

参考文献

ab93904 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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