リコンビナント
RabMAb

Anti-Ube2N / Ubc13 抗体 [EPR5162] (ab109286)

製品の概要

  • 製品名
    Anti-Ube2N / Ubc13 antibody [EPR5162]
    Ube2N / Ubc13 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5162] to Ube2N / Ubc13
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human Ube2N/ Ubc13

  • ポジティブ・コントロール
    • Human testis tissue. Jurkat, Daudi and Human heart lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 配列類似性
      Belongs to the ubiquitin-conjugating enzyme family.
    • 翻訳後修飾
      Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
    • 細胞内局在
      Nucleus. Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Bendless like ubiquitin conjugating enzyme antibody
      • Bendless-like ubiquitin-conjugating enzyme antibody
      • BLU antibody
      • EC 6.3.2.19 antibody
      • Epididymis secretory protein Li 71 antibody
      • HEL-S-71 antibody
      • Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds antibody
      • MGC131857 antibody
      • MGC8489 antibody
      • UBC 13 antibody
      • Ubc13 antibody
      • UbCH ben antibody
      • UbcH-ben antibody
      • UbcH13 antibody
      • UBCHBEN antibody
      • Ube 2N antibody
      • Ube2n antibody
      • UBE2N_HUMAN antibody
      • Ubiquitin carrier protein N antibody
      • Ubiquitin conjugating enzyme E2 N antibody
      • Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) antibody
      • Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast) antibody
      • Ubiquitin conjugating enzyme E2N antibody
      • Ubiquitin protein ligase N antibody
      • Ubiquitin-conjugating enzyme E2 N antibody
      • Ubiquitin-protein ligase N antibody
      • Yeast UBC13 homolog antibody
      see all

    画像

    • All lanes : Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : Human heart lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 17 kDa
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab109286 at 1/100 dilution.

    参考文献

    This product has been referenced in:
    • Wang C  et al. HTLV-1 Tax Functions as a Ubiquitin E3 Ligase for Direct IKK Activation via Synthesis of Mixed-Linkage Polyubiquitin Chains. PLoS Pathog 12:e1005584 (2016). Read more (PubMed: 27082114) »

    See 1 Publication for this product

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab109286.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録