製品の概要

  • 製品名Anti-Ube2N / Ubc13 antibody
    Ube2N / Ubc13 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Ube2N / Ubc13
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to 15 amino acids near the C terminus of Human Ube2N.

  • ポジティブ・コントロール
    • Human small intestine or HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 18 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 2 µg/ml.

ターゲット情報

  • 機能The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Belongs to the ubiquitin-conjugating enzyme family.
  • 翻訳後修飾Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
  • 細胞内局在Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bendless like ubiquitin conjugating enzyme antibody
    • Bendless-like ubiquitin-conjugating enzyme antibody
    • BLU antibody
    • EC 6.3.2.19 antibody
    • Epididymis secretory protein Li 71 antibody
    • HEL-S-71 antibody
    • Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds antibody
    • MGC131857 antibody
    • MGC8489 antibody
    • UBC 13 antibody
    • Ubc13 antibody
    • UbCH ben antibody
    • UbcH-ben antibody
    • UbcH13 antibody
    • UBCHBEN antibody
    • Ube 2N antibody
    • Ube2n antibody
    • UBE2N_HUMAN antibody
    • Ubiquitin carrier protein N antibody
    • Ubiquitin conjugating enzyme E2 N antibody
    • Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) antibody
    • Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2N antibody
    • Ubiquitin protein ligase N antibody
    • Ubiquitin-conjugating enzyme E2 N antibody
    • Ubiquitin-protein ligase N antibody
    • Yeast UBC13 homolog antibody
    see all

Anti-Ube2N / Ubc13 antibody 画像

  • All lanes : Anti-Ube2N / Ubc13 antibody (ab25885) (analysis in human small intestine cell lysates 15ug/lane )

    Lane 1 : 0.5 ug/ml of antibody.
    Lane 2 : 1 ug/ml of antibody.
    Lane 3 : 2 ug/ml of antibody.


    Predicted band size : 18 kDa
  • ab25885 at 2µg/ml staining UBe2N in mouse thymus tissue by IHC

  • ICC/IF image of ab25885 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25885, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Ube2N / Ubc13 antibody (ab25885) 使用論文

This product has been referenced in:
  • Kocevar N  et al. Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers. World J Gastroenterol 18:1216-28 (2012). WB ; Human . Read more (PubMed: 22468085) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (stomach)
Loading amount 30 µg
Specification stomach
Gel Running Conditions Reduced Denaturing (12 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"