Anti-UBE2M/UBC12 抗体 [EPR5334] (ab124946)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5334] to UBE2M/UBC12
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-UBE2M/UBC12 antibody [EPR5334]
UBE2M/UBC12 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5334] to UBE2M/UBC12 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human UBE2M/UBC12 aa 50-150. The exact sequence is proprietary.
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ポジティブ・コントロール
- Human 293T and Ramos cell line lysates
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5334 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124946の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 21 kDa. |
ターゲット情報
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機能
Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. -
パスウェイ
Protein modification; protein neddylation. -
配列類似性
Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily. -
ドメイン
Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8. - Information by UniProt
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参照データベース
- Entrez Gene: 9040 Human
- Entrez Gene: 22192 Mouse
- Entrez Gene: 361509 Rat
- Omim: 603173 Human
- SwissProt: P61081 Human
- SwissProt: P61082 Mouse
- Unigene: 406068 Human
- Unigene: 196580 Mouse
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別名
- hUbc12 antibody
- NEDD8 carrier protein antibody
- NEDD8 conjugating enzyme Ubc12 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab124946 は 1 報の論文で使用されています。
- Ba C et al. Ubiquitin conjugating enzyme E2 M promotes apoptosis in osteoarthritis chondrocytes via Wnt/ß-catenin signaling. Biochem Biophys Res Commun 529:970-976 (2020). PubMed: 32819607