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RabMAb

Anti-UBE2M 抗体 [EPR5333] (ab109507)

製品の概要

  • 製品名
    Anti-UBE2M antibody [EPR5333]
    UBE2M 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5333] to UBE2M
  • アプリケーション
    適用あり: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the C-terminus in Human UBE2M.

  • ポジティブ・コントロール
    • 293T and Ramos cell lysates, Human colonic adenocarcinoma and Human lung carcinoma tissues
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt 1/100 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.
  • パスウェイ
    Protein modification; protein neddylation.
  • 配列類似性
    Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily.
  • ドメイン
    Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hUbc12 antibody
    • NEDD8 carrier protein antibody
    • NEDD8 conjugating enzyme Ubc12 antibody
    • NEDD8 protein ligase antibody
    • NEDD8-conjugating enzyme Ubc12 antibody
    • UBC-RS2 antibody
    • UBC12 antibody
    • UBC12_HUMAN antibody
    • ube2m antibody
    • ubiquitin carrier protein M antibody
    • Ubiquitin conjugating enzyme E2 M antibody
    • Ubiquitin-conjugating enzyme E2 M antibody
    • ubiquitin-protein ligase M antibody
    • yeast UBC12 homolog antibody
    see all

画像

  • Overlay histogram showing Ramos cells stained with ab109507 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109507, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-UBE2M antibody [EPR5333] (ab109507) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : Ramos cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 21 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue using ab109507 at a dilution of 1/100.
  • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue using ab109507 at a dilution of 1/100.

参考文献

This product has been referenced in:
  • Coleman KE  et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Elife 6:N/A (2017). Read more (PubMed: 28475037) »
  • Xu J  et al. The neddylation-cullin 2-RBX1 E3 ligase axis targets tumor suppressor RhoB for degradation in liver cancer. Mol Cell Proteomics 14:499-509 (2015). Read more (PubMed: 25540389) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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