リコンビナント
RabMAb

Anti-Ube2L3 抗体 [EPR4368] (ab108936)

製品の概要

  • 製品名
    Anti-Ube2L3 antibody [EPR4368]
    Ube2L3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4368] to Ube2L3
  • アプリケーション
    適用あり: ICC/IF, WB, IP, IHC-Pmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Ube2L3

  • ポジティブ・コントロール
    • WB: HeLa, LNCaP, Jurkat and K562 cell lysates IHC-P: Human testis tissue IF: HeLa cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
    • 組織特異性
      Ubiquitous, with highest expression in testis.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 配列類似性
      Belongs to the ubiquitin-conjugating enzyme family.
    • 翻訳後修飾
      Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
    • 細胞内局在
      Nucleus. Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • E2 F1 antibody
      • L UBC antibody
      • L-UBC antibody
      • UB2L3_HUMAN antibody
      • UBCE7 antibody
      • UbcH7 antibody
      • UbcM4 antibody
      • Ube2l3 antibody
      • Ubiquitin carrier protein L3 antibody
      • Ubiquitin conjugating enzyme E2 L3 antibody
      • Ubiquitin protein ligase L3 antibody
      • Ubiquitin-conjugating enzyme E2 L3 antibody
      • Ubiquitin-conjugating enzyme E2-F1 antibody
      • Ubiquitin-protein ligase L3 antibody
      see all

    画像

    • All lanes : Anti-Ube2L3 antibody [EPR4368] (ab108936) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : LNCaP cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 18 kDa
    • ab108936, at a 1/100 dilution, staining Ube2L3 in paraffin-embedded Human testis tissue
    • ab108936, at a 1/100 dilution, staining Ube2L3 in HeLa cells

    参考文献

    This product has been referenced in:
    • Wang C  et al. HTLV-1 Tax Functions as a Ubiquitin E3 Ligase for Direct IKK Activation via Synthesis of Mixed-Linkage Polyubiquitin Chains. PLoS Pathog 12:e1005584 (2016). Read more (PubMed: 27082114) »

    See 1 Publication for this product

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab108936.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録