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Synthetic peptide: SLIALVNDPQ PEHPLRADLA EEYSKDRKKF CKNAEEFTKK YGEKRPVD, corresponding to C terminal amino acids 106-155 of Human Ube2L3
Our Abpromise guarantee covers the use of ab66944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analyisi of human intestine tissue labelling Ube2L3 with ab66944 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the intestinal villus. Magnification: 400X.
ab66944 has not yet been referenced specifically in any publications.