製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Ube2L3
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chicken, Cow, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide:

    C-EFTKKYGEKRPVD

    , corresponding to C terminal amino acids 142/154 of Human Ube2L3

  • ポジティブ・コントロール
    • Hela and Jurkat lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: antibody detection limit dilution 1/128000.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
    • 組織特異性
      Ubiquitous, with highest expression in testis.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 配列類似性
      Belongs to the ubiquitin-conjugating enzyme family.
    • 翻訳後修飾
      Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
    • 細胞内局在
      Nucleus. Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • E2 F1 antibody
      • L UBC antibody
      • L-UBC antibody
      • UB2L3_HUMAN antibody
      • UBCE7 antibody
      • UbcH7 antibody
      • UbcM4 antibody
      • Ube2l3 antibody
      • Ubiquitin carrier protein L3 antibody
      • Ubiquitin conjugating enzyme E2 L3 antibody
      • Ubiquitin protein ligase L3 antibody
      • Ubiquitin-conjugating enzyme E2 L3 antibody
      • Ubiquitin-conjugating enzyme E2-F1 antibody
      • Ubiquitin-protein ligase L3 antibody
      see all

    画像

    • All lanes : Anti-Ube2L3 antibody (ab37913) at 0.03 µg/ml

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : Mouse skeletal muscle lysate
      Lane 3 : Rat skeletal muscle lysate

      Lysates/proteins at 35 µg per lane.


      Predicted band size : 18 kDa
    • All lanes : Anti-Ube2L3 antibody (ab37913) at 0.01 µg/ml

      Lane 1 : Human heart lysate at 0.01 µg
      Lane 2 : Mouse heart lysate at 35 µg
      Lane 3 : Rat heart lysate at 35 µg
      Lane 4 : Pig heart lysate at 35 µg


      Predicted band size : 18 kDa
    • Anti-Ube2L3 antibody (ab37913) at 0.5 µg/ml + Hela lysate (RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
      Primary incubated for 1 hour.

    プロトコール

    参考文献

    This product has been referenced in:
    • Gandini MA  et al. The MAP1B-LC1/UBE2L3 complex catalyzes degradation of cell surface CaV2.2 channels. Channels (Austin) 8:452-7 (2014). Read more (PubMed: 25483588) »
    • Reyes-Hernández OD  et al. Ube2l3 gene expression is modulated by activation of the aryl hydrocarbon receptor: implications for p53 ubiquitination. Biochem Pharmacol 80:932-40 (2010). WB ; Mouse . Read more (PubMed: 20478272) »

    See all 2 Publications for this product

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