Ube2G1 overexpression 293T lysate (whole cell) (ab94306)

製品の概要

  • 製品名
    Ube2G1 overexpression 293T lysate (whole cell)
  • 製品の詳細
    Ube2G1 overexpression lysate
  • 特記事項
    ab94306 is a 293T cell transfected lysate in which Human Ube2G1 has been transiently over-expressed using a pCMV-Ube2G1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • アプリケーション
    適用あり: WBmore details

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • バッファー
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 精製度
    Whole Cell Lysate
  • 研究分野
  • 背景
    Function: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4. Tissue specificity: Widely expressed, mainly in skeletal muscle. Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the ubiquitin-conjugating enzyme family.

アプリケーション

Our Abpromise guarantee covers the use of ab94306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

画像

  • ab94306 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Ube2G1 antibody (ab58256) at 1/500 dilution

    Lane 1 : Ube2G1 overexpression 293T lysate (whole cell) (ab94306)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution

参考文献

ab94306 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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