製品の概要

  • 製品名
  • 製品の詳細
    Chicken polyclonal to UBE2D4
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein (Human)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 12 kDa). [A fusion protein was used to produce this Western Blot, hence the difference in M.Wt compared to the native protein.]


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 配列類似性
      Belongs to the ubiquitin-conjugating enzyme family.
    • Information by UniProt
    • 参照データベース
    • 別名
      • HBUCE 1 antibody
      • HBUCE1 antibody
      • UB2D4_HUMAN antibody
      • UBCH5D antibody
      • UBE2D 4 antibody
      • UBE2D4 antibody
      • Ubiquitin carrier protein D4 antibody
      • Ubiquitin conjugating enzyme 1 isoform antibody
      • Ubiquitin conjugating enzyme E2D 4 antibody
      • Ubiquitin conjugating enzyme HBUCE1 antibody
      • Ubiquitin-conjugating enzyme E2 D4 antibody
      • Ubiquitin-protein ligase D4 antibody
      see all

    画像

    • Anti-UBE2D4 antibody (ab37510) at 1/2000 dilution + E. coli-derived fusion protein at 2.5 µg

      Secondary
      Goat anti-IgY-HRP at 1/1000 dilution

      Predicted band size : 12 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    ab37510 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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