製品の概要
-
製品名Anti-Ube1L antibody [EPR4270]
Ube1L 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR4270] to Ube1L
-
由来種Rabbit
-
アプリケーション適用あり: WB, IP, Flow Cyt, ICC/IFmore details
適用なし: IHC-P -
種交差性交差種: Human
-
免疫原
Synthetic peptide within Human Ube1L (C terminal). The exact sequence is proprietary.
-
ポジティブ･コントロール
- HepG2, HL60, HeLa, Jurkat, and Human fetal liver cell lysates, HeLa cells
-
特記事項
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
-
精製度Tissue culture supernatant
-
ポリ/モノモノクローナル
-
クローン名EPR4270
-
アイソタイプIgG
-
研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab108929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa). | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
| ICC/IF | 1/100 - 1/250. |
ターゲット情報
-
機能Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
-
組織特異性Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
-
パスウェイProtein modification; protein ubiquitination.
-
配列類似性Belongs to the ubiquitin-activating E1 family.
- Information by UniProt
-
参照データベース
- Entrez Gene: 7318 Human
- Omim: 191325 Human
- SwissProt: P41226 Human
- Unigene: 16695 Human
-
別名
- D8 antibody
- MGC12713 antibody
- UBA1, ubiquitin activating enzyme E1 homolog B antibody
see all
画像
-
![Western blot - Anti-Ube1L antibody [EPR4270] (ab108929)](http://a.abcam.com/ps/products/108/ab108929/Images/Ube1L-Primary-antibodies-ab108929-2.JPG)
Western blot - Anti-Ube1L antibody [EPR4270] (ab108929)All lanes : Anti-Ube1L antibody [EPR4270] (ab108929) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 112 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Ube1L antibody [EPR4270] (ab108929)](http://a.abcam.com/ps/products/108/ab108929/Images/Ube1L-Primary-antibodies-ab108929-3.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Ube1L antibody [EPR4270] (ab108929)Immunofluorescent staining of HeLa cells using ab108929 at a dilution of 1/100.
プロトコール
参考文献
ab108929 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"