Anti-Ube1L 抗体 [EPR4270] (ab108929)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4270] to Ube1L
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Ube1L antibody [EPR4270]
Ube1L 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4270] to Ube1L -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HepG2, HL60, HeLa, Jurkat, and human fetal liver cell lysate. GST tagged Recombinant human UBA7(Ube1L) protein. ICC/IF: HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR4270 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108929の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
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ICC/IF |
1/100 - 1/250.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa). |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. -
組織特異性
Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the ubiquitin-activating E1 family. - Information by UniProt
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参照データベース
- Entrez Gene: 7318 Human
- Omim: 191325 Human
- SwissProt: P41226 Human
- Unigene: 16695 Human
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別名
- D8 antibody
- MGC12713 antibody
- UBA1, ubiquitin activating enzyme E1 homolog B antibody
see all
画像
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All lanes : Anti-Ube1L antibody [EPR4270] (ab108929) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HL60 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 112 kDa -
All lanes : Anti-Ube1L antibody [EPR4270] (ab108929) at 1/1000 dilution
Lane 1 : GST tagged Recombinant human UBA7(Ube1L) protein (Full length, 166 KDa)
Lane 2 : GST tagged Recombinant human UBA1 protein (Full length, 146 KDa)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 112 kDa
Observed band size: 146,166 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
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Immunofluorescent staining of HeLa cells using ab108929 at a dilution of 1/100.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab108929 は論文での使用が確認できていません。