Rabbit polyclonal to UBAP2L
Synthetic peptide corresponding to a region between residues 400 and 450 of human UBAP2L (AAH03170.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at a concentration of 2 - 5 µg/mg.
WB: 1/5000 - 1/25000. Detects a band of approximately 160 kDa (predicted molecular weight: 115 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Contains 1 UBA domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
NICE 4 antibody
Western blot - Anti-UBAP2L antibody (ab70319)
All lanes :
Anti-UBAP2L antibody (ab70319) at 0.03 µg/ml
Lane 1 :
Mock treated Hela cell lysate
Lane 2 :
Hela cell lysate treated with a control siRNA
Lane 3 :
Hela cell lysate treated with siRNA for UBAP2L
Lysates/proteins at 100 µg per lane.
Predicted band size:
Observed band size:
160 kDa (
why is the actual band size different from the predicted?
Additional bands at:
50 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Detection: Chemiluminescence with an exposure time of 15 seconds.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"