Anti-uPA Receptor/U-PAR 抗体 (ab103791)
Key features and details
- Rabbit polyclonal to uPA Receptor/U-PAR
- Suitable for: WB, ICC
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-uPA Receptor/U-PAR antibody
uPA Receptor/U-PAR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to uPA Receptor/U-PAR -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab103791の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.
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ICC |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. |
ICC
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form. -
組織特異性
Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain. -
配列類似性
Contains 3 UPAR/Ly6 domains. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5329 Human
- Entrez Gene: 50692 Rat
- Omim: 173391 Human
- SwissProt: Q03405 Human
- SwissProt: P49616 Rat
- Unigene: 466871 Human
- Unigene: 82711 Rat
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別名
- CD 87 antibody
- CD87 antibody
- CD87 antigen antibody
see all
画像
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All lanes : Anti-uPA Receptor/U-PAR antibody (ab103791) at 1 µg/ml
Lane 1 : Human placenta tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat thymus tissue lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : MCF7 whole cell lysate
Lane 6 : SMMC whole cell lysate
Lane 7 : HeLa whole cell lysate
Lane 8 : Raji whole cell lysate
Lane 9 : Colo320 whole cell lysate
Predicted band size: 37 kDa -
ab103791, at 1µg/ml, staining uPA Receptor/U-PAR in MCF7 cells by Immunocytochemistry.
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MDA-MB-231 cells were incubated at 37°C for 24h with vehicle control (0 µM) and varied concentrations of SB 203580 hydrochloride (ab120235). Decreased expression of uPA receptor/U-PAR in MDA-MB-231 cells correlates with an increase in SB 203580 hydrochloride concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab103791 at 1 µg/ml and ab1801 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab103791 は 10 報の論文で使用されています。
- Fu X et al. Molecular mechanism of albumin in suppressing invasion and metastasis of hepatocellular carcinoma. Liver Int 42:696-709 (2022). PubMed: 34854209
- Abufaraj M et al. The expression of urokinase-type plasminogen activator system in upper tract urothelial carcinoma and its prognostic value after radical nephroureterectomy. Urol Oncol N/A:N/A (2020). PubMed: 32381392
- Gu C et al. Upregulation of miR-324-5p Inhibits Proliferation and Invasion of Colorectal Cancer Cells by Targeting ELAVL1. Oncol Res 27:515-524 (2019). PubMed: 29386086
- Cammalleri M et al. The urokinase-type plasminogen activator system as drug target in retinitis pigmentosa: New pre-clinical evidence in the rd10 mouse model. J Cell Mol Med 23:5176-5192 (2019). PubMed: 31251468
- Wach S et al. Exploring the MIR143-UPAR Axis for the Inhibition of Human Prostate Cancer Cells In Vitro and In Vivo. Mol Ther Nucleic Acids 16:272-283 (2019). PubMed: 30933831
- Huang JM et al. Ulinastatin Inhibits Osteoclastogenesis and Suppresses Ovariectomy-Induced Bone Loss by Downregulating uPAR. Front Pharmacol 9:1016 (2018). PubMed: 30245631
- Shang X et al. Serine protease PRSS55 is crucial for male mouse fertility via affecting sperm migration and sperm-egg binding. Cell Mol Life Sci N/A:N/A (2018). PubMed: 30032357
- Hao XD et al. De novo mutations of TUBA3D are associated with keratoconus. Sci Rep 7:13570 (2017). PubMed: 29051577
- Puthusseri B et al. Regulation of p53-mediated changes in the uPA-fibrinolytic system and in lung injury by loss of surfactant protein C expression in alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol 312:L783-L796 (2017). PubMed: 28385810
- Al Dhaheri Y et al. Anti-metastatic and anti-tumor growth effects of Origanum majorana on highly metastatic human breast cancer cells: inhibition of NF?B signaling and reduction of nitric oxide production. PLoS One 8:e68808 (2013). WB . PubMed: 23874773