製品の概要
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製品名U937 nuclear extract lysate (IFN alpha treated)
U937 ライゼート 製品一覧 -
製品の詳細U937 nuclear extract lysate
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特記事項Cells were cultured in medium supplemented with 5000 U/mL interferon alpha for one hour at 37°C immediately prior to harvesting. Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
製品の特性
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製品の状態Liquid
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保存方法Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9 -
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研究分野
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背景The U937 cell line is a human cell line established from a diffuse histiocytic lymphoma and displaying many monocytic characteristics. It serves as an in vitro model for monocyte/macrophage differentiation, antitumour testing, tumorigenicity. U937 cells dominantly expressed the 44-46 kDa paxillin forms and IFN-beta and IFN-gamma stimulation lead to the accumulation of 66-70 kDa paxillin forms.
U937 nuclear extract lysate (IFN alpha treated) (ab14907) 使用論文
ab14907 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"