U937 nuclear extract lysate (IFN alpha treated) (ab14907)

Q&A (1)

製品の概要

  • 製品名
    U937 nuclear extract lysate (IFN alpha treated)
    U937 ライゼート 製品一覧
  • 製品の詳細
    U937 nuclear extract lysate
  • 特記事項
    Cells were cultured in medium supplemented with 5000 U/mL interferon alpha for one hour at 37°C immediately prior to harvesting. Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
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  • 研究分野
  • 背景
    The U937 cell line is a human cell line established from a diffuse histiocytic lymphoma and displaying many monocytic characteristics. It serves as an in vitro model for monocyte/macrophage differentiation, antitumour testing, tumorigenicity. U937 cells dominantly expressed the 44-46 kDa paxillin forms and IFN-beta and IFN-gamma stimulation lead to the accumulation of 66-70 kDa paxillin forms.

参考文献

ab14907 has not yet been referenced specifically in any publications.

ab14907 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

質問を送る

1 Q&A

10
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Why were the cells harvested immediately after IFNalpha treatment? Do you have a reference explaining that this is necessary to maintain the integrity of the lysate proteins? If I am using the lysate in Western blot, do I need to handle them differentl...

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Thank you for your phone call the other day. I have heard back from the production lab regarding the IFN alpha treatment of the cell prior to lysis, and I received the following response: "The treatment with IFN alpha has no effect on the stabili...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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