製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to U2AF1L3
  • 特異性
    ab80607 is expected to recognise isoforms 1 and 2 (NP_001035515.1 and NP_659424.2). No cross-reactivity with U2AF1 expected.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide:

    C-EDGERAVAELSNR

    , corresponding to internal sequence amino acids 121 - 133 of Human U2AF1L3 (NP_001035515.1 and NP_659424.2)

  • ポジティブ・コントロール
    • Human Cerebellum lysate and lung lysates

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab80607 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab80607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    Peptide ELISA: antibody detection limit dilution 1/32000
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 26 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T cell activation; an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site.
    • 組織特異性
      Isoform 2 is widely expressed. Isoform 3 is highly expressed in heart, brain and lung, lower expressed in thymus and much lower expressed in peripheral blood leukocytes.
    • 配列類似性
      Belongs to the splicing factor SR family.
      Contains 2 C3H1-type zinc fingers.
      Contains 1 RRM (RNA recognition motif) domain.
    • ドメイン
      The second zinc finger in necessary for interaction with GFI1 and for alternative pre-mRNA splicing events.
    • 細胞内局在
      Nucleus. Nucleus speckle.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ35525 antibody
      • MGC33901 antibody
      • Splicing factor U2AF 26 kDa subunit antibody
      • U2 auxiliary factor 26 antibody
      • U2 small nuclear RNA auxiliary factor 1 like 3 antibody
      • U2 small nuclear RNA auxiliary factor 1 like 4 antibody
      • U2 small nuclear RNA auxiliary factor 1 like protein 3 antibody
      • U2 small nuclear RNA auxiliary factor 1 like protein 4 antibody
      • U2 small nuclear RNA auxiliary factor 1-like protein 3 antibody
      • U2 small nuclear RNA auxiliary factor 1-like protein 4 antibody
      • U2(RNU2) small nuclear RNA auxiliary factor 1 like 3 antibody
      • U2(RNU2) small nuclear RNA auxiliary factor 1-like protein 3 antibody
      • U2AF1 like protein 3 antibody
      • U2AF1 RS3 antibody
      • U2AF1-like protein 3 antibody
      • U2AF1L3V1 antibody
      • U2AF1L4 antibody
      • U2AF1RS3 antibody
      • U2af26 antibody
      • U2AF4_HUMAN antibody
      • U2RNU2 small nuclear RNA auxiliary factor 1 like 3 antibody
      • U2RNU2 small nuclear RNA auxiliary factor 1 like protein 3 antibody
      see all

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    プロトコール

    参考文献

    ab80607 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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