The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Titre using peptide based assay 1/1562500.
Use a concentration of 4 - 8 µg/ml.
U1SNRNPBP is a homolog of U1-snRNP binding protein. Its N-terminal contains an RNA recognition motif. The C-terminal is rich in Arg/Asp and Arg/Glu dipeptides, commonly found in splicing factors. Exists in at least two isoforms produced by alternative splicing that differ in the 5' sequence regions.
Shen H et al. The U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing. Genes Dev24:2389-94 (2010).
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