製品の概要

  • 製品名Anti-U1A antibody
    U1A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to U1A
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-283 of Human U1A

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab55751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 31 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.
  • 配列類似性Belongs to the RRM U1 A/B'' family.
    Contains 2 RRM (RNA recognition motif) domains.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • fc19d01 antibody
    • Mud1 antibody
    • small nuclear ribonucleoprotein polypeptide A antibody
    • snRNP A antibody
    • snRNP protein A antibody
    • SNRPA antibody
    • SNRPA_HUMAN antibody
    • U1 small nuclear ribonucleoprotein A antibody
    • U1 small nuclear RNP specific A antibody
    • U1 snRNP A antibody
    • U1 snRNP specific protein A antibody
    • U1-A antibody
    • U1A antibody
    • wu:fc19d01 antibody
    • zgc:77810 antibody
    see all

Anti-U1A antibody 画像

  • U1A antibody (ab55751) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart tissue.


  • Predicted band size : 31 kDa
    U1A antibody (ab55751) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55751 at 10 ug/ml staining U1A in human Hella cells by Immunocytochemistry/ Immunofluorescence.

  • Overlay histogram showing HeLa cells stained with ab55751 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55751, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-U1A antibody (ab55751) 使用論文

This product has been referenced in:
  • Llorian M  et al. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Res 44:8933-8950 (2016). WB ; Mouse . Read more (PubMed: 27317697) »
  • Ammon T  et al. The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells. J Mol Cell Biol 6:312-23 (2014). Human . Read more (PubMed: 24872507) »

See all 4 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Nupage gel)
Sample Mouse Cell lysate - whole cell (NSC34 cells)
Specification NSC34 cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Cheng Song

Verified customer

投稿 Aug 26 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"