This antibody gave a positive signal in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line)
Jurkat (Human T cell lymphoblast-like cell line)
A431 (Human epithelial carcinoma cell line)
HEK293 (Human embryonic kidney cell line)
MCF7 (Human breast adenocarcinoma cell line)
SHSY-5Y (Human neuroblastoma cell line)
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
Use a concentration of 5 µg/ml.
Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.
Belongs to the RRM U1 A/B'' family. Contains 2 RRM (RNA recognition motif) domains.
ICC/IF image of ab40689 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40689, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - Anti-U1A antibody (ab40689)
Anti-U1A antibody (ab40689) at 1/200 dilution + NSC34 cell nuclear extract at 20 µg
Secondary IRDye® 800CW Donkey anti-Rabbit IgG at 1/3000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 31 kDa Observed band size : 31 kDa