Standard phospholipase C inhibitor (PLC) (IC50 = ~6 µM at PLC-β3). Shows a variety of biological activities including antiplatelet activation, Ca2+ signaling modulation and inhibition of 5-lipoxygenase activity. Active in vivo.
Store at Room Temperature. The product can be stored for up to 12 months.
Soluble in DMSO to 10 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Liang WZ et al. The mechanism of honokiol-induced intracellular Ca(2+) rises and apoptosis in human glioblastoma cells. Chem Biol Interact221C:13-23 (2014).
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Shen W et al. The phospholipase C inhibitor U73122 attenuates trans-10, cis-12 conjugated linoleic acid-mediated inflammatory signaling and insulin resistance in human adipocytes. J Nutr143:584-90 (2013).
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Burgdorf C et al. U73122, an aminosteroid phospholipase C inhibitor, is a potent inhibitor of cardiac phospholipase D by a PIP2-dependent mechanism. J Cardiovasc Pharmacol55:555-9 (2010).
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Wilsher NE et al. The phosphoinositide-specific phospholipase C inhibitor U73122 (1-(6-((17beta-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione) spontaneously forms conjugates with common components of cell culture medium. Drug Metab Dispos35:1017-22 (2007).
Read more (PubMed: 17403917) »
Hou C et al. In vivo activity of a phospholipase C inhibitor, 1-(6-((17beta-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione (U73122), in acute and chronic inflammatory reactions. J Pharmacol Exp Ther309:697-704 (2004).
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Muto Y et al. The putative phospholipase C inhibitor U73122 and its negative control, U73343, elicit unexpected effects on the rabbit parietal cell. J Pharmacol Exp Ther282:1379-88 (1997).
Read more (PubMed: 9316850) »