製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to TZFP
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length human TZFP with proprietary tag, amino acids 1-302 (AAH17700.1)

  • ポジティブ・コントロール
    • Human colon tissue lysate, TZFP transfected 293T cell lysate, HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T cells.
  • 組織特異性
    Predominantly expressed in testis. Some isoforms are ubiquitously expressed.
  • 配列類似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 3 C2H2-type zinc fingers.
  • ドメイン
    The C-terminal zinc finger domain functions as a transcriptional transactivator.
    The BTB (POZ) domain possesses repressor activity.
  • 細胞内局在
    Nucleus. Located in nuclear speckles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FANCC interacting protein antibody
    • FANCC-interacting protein antibody
    • Fanconi anemia zinc finger protein antibody
    • FAXF antibody
    • FAZF antibody
    • OTTHUMP00000045790 antibody
    • repressor of GATA antibody
    • Rog antibody
    • Testis zinc finger protein antibody
    • ZBT32_HUMAN antibody
    • Zbtb32 antibody
    • ZBTB32 protein antibody
    • Zinc finger and BTB domain containing 32 antibody
    • Zinc finger and BTB domain-containing protein 32 antibody
    • Zinc finger protein 538 antibody
    • ZNF538 antibody
    see all

画像

  • Anti-TZFP antibody (ab88594) at 5 µg/ml + Human colon tissue lysate at 50 µg

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • All lanes : Anti-TZFP antibody (ab88594) at 5 µg/ml

    Lane 1 : TZFP transfected 293t cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg/ml per lane.


    Predicted band size : 53 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • ab88594 at 10µg/ml staining TZFP in HeLa cells by Immunofluorescence.

参考文献

ab88594 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (transfected 293T cells)
Specification
transfected 293T cells
Total protein in input
2e+006 cells
Username

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投稿 Feb 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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