製品の概要

  • 製品名Anti-TZFP antibody
    TZFP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TZFP
  • 特異性This antibody recognizes TZFP
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Chimpanzee
  • 免疫原

    A region within synthetic peptide: PIRLPSPYGS DRLVQLAARL RPALCDTLIT VGSQEFPAHS LVLAGVSQQL, corresponding to amino acids 5-54 of Human TZFP

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab49349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:12500.

ターゲット情報

  • 機能DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T cells.
  • 組織特異性Predominantly expressed in testis. Some isoforms are ubiquitously expressed.
  • 配列類似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 3 C2H2-type zinc fingers.
  • ドメインThe C-terminal zinc finger domain functions as a transcriptional transactivator.
    The BTB (POZ) domain possesses repressor activity.
  • 細胞内局在Nucleus. Located in nuclear speckles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FANCC interacting protein antibody
    • FANCC-interacting protein antibody
    • Fanconi anemia zinc finger protein antibody
    • FAXF antibody
    • FAZF antibody
    • OTTHUMP00000045790 antibody
    • repressor of GATA antibody
    • Rog antibody
    • Testis zinc finger protein antibody
    • ZBT32_HUMAN antibody
    • Zbtb32 antibody
    • ZBTB32 protein antibody
    • Zinc finger and BTB domain containing 32 antibody
    • Zinc finger and BTB domain-containing protein 32 antibody
    • Zinc finger protein 538 antibody
    • ZNF538 antibody
    see all

Anti-TZFP antibody 画像

  • Anti-TZFP antibody (ab49349) at 0.5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 33 kDa

Anti-TZFP antibody (ab49349) 使用論文

ab49349 has not yet been referenced specifically in any publications.

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