Anti-Tyrosyl tRNA synthetase 抗体 [YAR5H08] (ab50961)

製品の概要

  • 製品名Anti-Tyrosyl tRNA synthetase antibody [YAR5H08]
    Tyrosyl tRNA synthetase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [YAR5H08] to Tyrosyl tRNA synthetase
  • アプリケーション適用あり: WB, IP, ICC, Dot blotmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant human YARS fragment

  • ポジティブ・コントロール
    • HeLa cells and cell lysate and NIH3T3 cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM KH2PO4, 8.0mM Na2HPO4, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)ab50961 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • ポリ/モノモノクローナル
  • クローン名YAR5H08
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IP Use at 20 µg/mg of lysate.
ICC 1/10.
Dot blot Use at an assay dependent dilution.

ターゲット情報

  • 機能Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
  • 関連疾患Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • 配列類似性Belongs to the class-I aminoacyl-tRNA synthetase family.
    Contains 1 tRNA-binding domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CMTDIC antibody
    • SYYC_HUMAN antibody
    • Tyrosine tRNA ligase, cytoplasmic antibody
    • Tyrosine tRNA ligase 1, cytoplasmic antibody
    • Tyrosyl tRNA synthetase antibody
    • Tyrosyl--tRNA ligase antibody
    • Tyrosyl-tRNA synthetase, cytoplasmic antibody
    • TyrRS antibody
    • yars antibody
    • YRS antibody
    • YTS antibody
    see all

Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] 画像

  • Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] (ab50961) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • Immunoprecipititation analysis using 10ug ab50961 per 500ug RIPA HeLa cell lysate. Immunoblot using ab50961 at 1/100 dilution and TrueBlotTM anti-mouse IgG at 1/1000 as secondary antibody.
  • Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] (ab50961) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ab50961 at 1/10 dilution staining human YARS in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat anti-mouse IgG at 1/200 as secondary.

Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] (ab50961) 使用論文

This product has been referenced in:
  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"