製品の概要

  • 製品名Anti-Tyrosine Hydroxylase antibody
    Tyrosine Hydroxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Tyrosine Hydroxylase
  • 特異性Immunohistochemical distribution throughout the brain shows that staining is restricted to these neurons that are known to contain the enzyme.
  • アプリケーション適用あり: IHC-FoFr, ELISA, WB, ICC/IF, IHC (PFA fixed), IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Guinea pig, Human
    交差が予測される動物種: Chicken, Chimpanzee, Macaque Monkey
  • 免疫原

    Synthetic peptide corresponding to Rat Tyrosine Hydroxylase aa 32-47 conjugated to Keyhole Limpet Haemocyanin (KLH) (Glutaraldehyde).
    Sequence:

    PRFIGRRQSLIEDARK


    Database link: P04177

  • ポジティブ・コントロール
    • Mouse or rat brain containing dopaminergic neurons. Ventral mesencephalic neurons (mouse embryonic day 14).
  • 特記事項

     

    This antibody has proven useful in staining catecholaminergic neurons. It is a high quality reagent that stains these neurons intensely, including dendritic processes and fine nerve terminals.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/500. PubMed: 19630993
ELISA Use at an assay dependent concentration.
WB 1/100 - 1/500. Predicted molecular weight: 42,53,84 kDa.
ICC/IF 1/1000.
IHC (PFA fixed) 1/1000.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能Plays an important role in the physiology of adrenergic neurons.
  • 組織特異性Mainly expressed in the brain and adrenal glands.
  • パスウェイCatecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • 関連疾患Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • 配列類似性Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Anti-Tyrosine Hydroxylase antibody 画像

  • Tyrosine hydroxylase antibody (ab6211) staining rat mesencephalic serotoninergic neurons. Pictures taken using objectives X20 (A) or X20 (B). Note that the staining is observed in the cell body and the processes of these neurons. ab6211 was used at 1/1000 incubated for 2 days at room temperature. Secondary antibody was anti-rabbit Alexa Fluor 488 used at 1/1000 for 2 hours at room temperature. Rat brain tissue (ab29475) was perfusion fixed (paraformaldehyde 4% ) followed by post-fixation overnight in the same fixative, cryoprotected in 20% sucrose and then frozen in OCT. 30µm coronal sections of brain were cutting by cyrostat and immunohistochemistry was performed as the 'free floating' technique.

  • ab6211 staining tyrosine hydroxylase in PC-12 cells treated with orexin A (ab120212), by ICC/IF. Decrease in expression of tyrosine hydroxylase correlates with increased concentration of orexin A, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120212 (orexin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum (ab7481), 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6211 (1/1000 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

  • ab6211 Rabbit polyclonal to Tyrosine Hydroxylase (1/1000) on cryopreserved ventral mesencephalic neurons dissected at mouse embryonic day 14. Neurons were thawed, plated and grown for 14 days, before fixation and overnight staining with ab6211.
  • Immunohistochemical detection of dopaminergic neurons in the rat zona incerta in a formalin-fixed floated cryostat section. Tyrosine hydroxylase immunoreactivity was visualized with ab6211 (1:100,000), using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

  • Immunohistochemical staining of catecholaminergic neurons in the rat brain stem.

  • Immunohistochemical staining of catecholaminergic neurons in the rat brain stem.

Anti-Tyrosine Hydroxylase antibody (ab6211) 使用論文

This product has been referenced in:
  • Peng X  et al. Germline transmission of an embryonic stem cell line derived from BALB/c cataract mice. PLoS One 9:e90707 (2014). ICC/IF ; Mouse . Read more (PubMed: 24595217) »
  • Guo S  et al. Optogenetic activation of the excitatory neurons expressing CaMKIIa in the ventral tegmental area upregulates the locomotor activity of free behaving rats. Biomed Res Int 2014:687469 (2014). IHC ; Rat . Read more (PubMed: 24711999) »

See all 14 Publications for this product

Product Wall

Application Immunohistochemistry (Resin sections)
Sample Mouse Tissue sections (Retina)
Specification Retina
Username

Abcam user community

Verified customer

投稿 Jun 27 2016

Thank you for contacting Abcam.

I apologize for the delay, our laboratory was looking into the availability of the blocking peptide for ab6211.

We do not have the blocking peptide for ab6211 available separately. However, the peptide ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain-SNpc)
Specification Brain-SNpc
Fixative Formaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Apr 13 2012

Thank you for confirming this information and for your help and cooperation with this case. As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order. As usual ...

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Thank you for filling in the questionnaire and for reporting this problem to us. We will use the details you sent about your protocol in order to investigate the source of the problem. I have looked through your protocol, which looks fine to me. I a...

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Thank you for your enquiry. Since you are seeing some specific staining in addition to this ubiquitous background staining, I am thinking this may be non-specific staining due to using too high a concentration of primary antibody. The recommendatio...

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Thank you for your enquiry. Regarding "using protein in the phenol-ethanol supernatant for ELISA", would you first precipitate the protein? In either case, I'm not sure if the protein would be recognized by the antibodies since we have not tested t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Rat mesencephalic brain)
Specification Rat mesencephalic brain
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

投稿 Apr 12 2006

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soona s any new information is brought to our attention. As far as we are aware, cross reactivity with human has...

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There are no preservatives present in the whole serum. The whole antiserum was just sterilized by pushing it through a 0.2 um amicon filter.

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