製品の概要

  • 製品名Anti-Tyrosine Hydroxylase antibody
    Tyrosine Hydroxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Tyrosine Hydroxylase
  • 特異性This antibody detects endogenous levels of total Tyrosine Hydroxylase protein.
  • アプリケーション適用あり: IHC-FoFr, ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthesized non-phosphopeptide derived from Tyrosine Hydroxylase around the phosphorylation site of Serine 19 (AVSPEQ) (Human)

  • ポジティブ・コントロール
    • Extracts from 3T3 cells treated with Tyrosine Hydroxylase antibody; Human brain tissue

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab51191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 20923695
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/300 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 59 kDa).

ターゲット情報

  • 機能Plays an important role in the physiology of adrenergic neurons.
  • 組織特異性Mainly expressed in the brain and adrenal glands.
  • パスウェイCatecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • 関連疾患Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • 配列類似性Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Anti-Tyrosine Hydroxylase antibody 画像

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab51191 (1/50 dilution). Left: without immunizing peptide, Right: with immunizing peptide
  • All lanes : Anti-Tyrosine Hydroxylase antibody (ab51191) at 1/300 dilution

    Lane 1 : Extracts from 3T3 cells treated with Forskolin (40nM, 30min) with no peptide
    Lane 2 : Extracts from 3T3 cells treated with Forskolin (40nM, 30min) with peptide


    Predicted band size : 59 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

Anti-Tyrosine Hydroxylase antibody (ab51191) 使用論文

This product has been referenced in:
  • Chung K  et al. Structural and molecular interrogation of intact biological systems. Nature 497:332-7 (2013). Read more (PubMed: 23575631) »
  • Cambon K  et al. Aggressive behavior during social interaction in mice is controlled by the modulation of tyrosine hydroxylase expression in the prefrontal cortex. Neuroscience 171:840-51 (2010). IHC-FoFr ; Mouse . Read more (PubMed: 20923695) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam.

The blocking peptide for ab51191 has been published as Tyrosine Hydroxylase peptide (ab151686):

http://www.abcam.com/tyrosine-hydroxylase-peptide-ab151686.html



Please let me know ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain)
Loading amount 30 µg
Specification Brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

投稿 Apr 16 2012

Thank you for your inquiry.
We do guarantee both of these antibodies, ab51191 and ab112, to work on rat IHC-P. Therefore both should be adapted for your study. The ab112 has been used up to 1/750 in IHC-P and has been produced against the full len...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"