製品の概要

  • 製品名Anti-Tyrosine Hydroxylase antibody
    Tyrosine Hydroxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Tyrosine Hydroxylase
  • アプリケーション適用あり: IHC-Fr, IHC-P, IHC-FrFl, ICC/IF, IHC-FoFr, WB, IP, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Cat, Human
    交差が予測される動物種: all Mammals
  • 免疫原

    Full length SDS denatured protein (purified from pheochromocytoma) (Rat).

  • 特記事項

    ab112 can be used as a marker for dopaminergic and noradrenergic neurons.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/750 - 1/1500.
IHC-P 1/750.
IHC-FrFl 1/1000. PubMed: 222726
ICC/IF 1/1000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20357108
WB 1/200. Predicted molecular weight: 60 kDa.
IP Use at an assay dependent concentration.
ICC 1/2000.

ターゲット情報

  • 機能Plays an important role in the physiology of adrenergic neurons.
  • 組織特異性Mainly expressed in the brain and adrenal glands.
  • パスウェイCatecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • 関連疾患Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • 配列類似性Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Anti-Tyrosine Hydroxylase antibody 画像

  • ab112 staining Tyrosine Hydroxylase in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/800 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody. The image of Caudate putamen shows characteristic nerve fibre positivity

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  • Predicted band size : 60 kDa

    Western Blot: ab112 - WB analysis of Tyrosine Hydroxylase in PC12 cell whole lysate.

  • ab112 staining tyrosine hydroxulase in mouse brain tissue. Sections were permeabilized with 0.5% Triton X-100 in PBS at room temperature for 10 minutes. Samples were incubated with primary antibody (1:500) overnight at 4C.

  • ab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry. The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.

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  • Predicted band size : 60 kDa

    Western Blot: ab112 - 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k TH protein.

  • ab112 at 1/500 staining rat brain tissue (ab29475) sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 14 hours. A biotinylated swine anti-rabbit IgG was used as the secondary.

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  • ab112, at 1/750, staining tyrosine hydroxylase in mouse brain tissue (ab30151) by Immunohistochemistry (Frozen sections). Sections were PFA fixed, permeabilized in 0.3 Triton X-100 prior to blocking in 5% serum for 1 hour at 22°C and then incubated with ab112, for 16 hours at 22°C. Alexa fluor® 555 goat polyclonal to rabbit Ig (ab150078), diluted 1/1000, was used as the secondary antibody.

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  • Immunohistochemical staining of retina tissue using ab112.

Anti-Tyrosine Hydroxylase antibody (ab112) 使用論文

This product has been referenced in:
  • Nandi SS  et al. Lack of miR-133a Decreases Contractility of Diabetic Hearts: A Role for Novel Cross Talk Between Tyrosine Aminotransferase and Tyrosine Hydroxylase. Diabetes 65:3075-90 (2016). Read more (PubMed: 27411382) »
  • Burré J  et al. Definition of a molecular pathway mediating a-synuclein neurotoxicity. J Neurosci 35:5221-32 (2015). Read more (PubMed: 25834048) »

See all 30 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain)
Permeabilization No
Specification brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Mr. Tamas Bellak

Verified customer

投稿 Aug 29 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - Triton x-100, 0.01%
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
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投稿 Jun 18 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Mouse Tissue sections (heart)
Specification heart
Permeabilization Yes - Triton x-100, 0.01%
Fixative Paraformaldehyde
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投稿 Feb 13 2015

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (CATH.a neuron)
Specification CATH.a neuron
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Jan 30 2015

Application Immunocytochemistry
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Sample Mouse Cultured Cells (CATH.a neuron)
Specification CATH.a neuron
Permeabilization Yes - Triton x-100, 0.01%
Fixative Formaldehyde
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投稿 Jan 16 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Oct 06 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Aug 10 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (brain cell line)
Specification brain cell line
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
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投稿 Jan 31 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Specification kidney
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 18 2012

Thank you for your email. We apologize for the inconvenience.

The extra antibody can be stored neat at -20C and will be viable for 10 years. The viability of reconstituted/ diluted antibody is less than neat due to the problem of ice crystalli...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"