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Recombinant Tyrosinase protein.
Clone T311 is a useful marker for melanocytes and melanomas.
Our Abpromise guarantee covers the use of ab79673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 60 kDa.
Use at a concentration of 1 - 2 µg/ml for 2hrs at RT.
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use at a concentration of 2 - 4 µg/ml for 30min at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
|ICC/IF||Use at an assay dependent concentration.|