製品の概要
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製品名Anti-Tyrosinase antibody
Tyrosinase 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Tyrosinase
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由来種Rabbit
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アプリケーション適用あり: ICC/IF, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee
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免疫原
A region within synthetic peptide: TQYESGSMDK AANFSFRNTL EGFASPLTGI ADASQSSMHN ALHIYMNGTM, corresponding to internal sequence amino acids 325-374 of Human Tyrosinase
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ポジティブ･コントロール
- Jurkat cell lysate. IF/ICC: Malme-3M cell line.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab61294 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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機能This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
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関連疾患Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C. -
配列類似性Belongs to the tyrosinase family.
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細胞内局在Melanosome membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 280951 Cow
- Entrez Gene: 7299 Human
- Entrez Gene: 22173 Mouse
- Entrez Gene: 308800 Rat
- Omim: 606933 Human
- SwissProt: Q8MIU0 Cow
- SwissProt: P54834 Dog
- SwissProt: P14679 Human
see all -
別名
- ATN antibody
- CMM8 antibody
- LB24 AB antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Tyrosinase antibody (ab61294)ICC/IF image of ab61294 stained Malme-3M cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61294, 5µg/ml) overnight at +4°C. The secondary antibody (green) was a96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Tyrosinase antibody (ab61294)Anti-Tyrosinase antibody (ab61294) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
参考文献
This product has been referenced in:
- Cao J et al. Tuberous sclerosis complex inactivation disrupts melanogenesis via mTORC1 activation. J Clin Invest 127:349-364 (2017). Read more (PubMed: 27918305) »
- Wang Q et al. Stress-induced RNASET2 overexpression mediates melanocyte apoptosis via the TRAF2 pathway in vitro. Cell Death Dis 5:e1022 (2014). WB . Read more (PubMed: 24457966) »
レビューと Q&A
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