製品の概要

  • 製品名Anti-Tyrosinase antibody
    Tyrosinase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Tyrosinase
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee
  • 免疫原

    A region within synthetic peptide: TQYESGSMDK AANFSFRNTL EGFASPLTGI ADASQSSMHN ALHIYMNGTM, corresponding to internal sequence amino acids 325-374 of Human Tyrosinase

  • ポジティブ・コントロール
    • Jurkat cell lysate. IF/ICC: Malme-3M cell line.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab61294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone.
  • 関連疾患Defects in TYR are the cause of albinism oculocutaneous type 1A (OCA1A) [MIM:203100]; also known as tyrosinase negative oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is absent in skin, hair, and eyes. It is characterized by complete lack of tyrosinase activity due to production of an inactive enzyme. Patients present with a life-long absence of melanin pigment after birth, and manifest increased sensitivity to ultraviolet radiation with predisposition to skin cancer. Visual anomalies include decreased acuity, nystagmus, strabismus and photophobia.
    Defects in TYR are the cause of albinism oculocutaneous type 1B (OCA1B) [MIM:606952]; also known as albinism yellow mutant type. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. It is characterized by partial lack of tyrosinase activity. Patients have white hair at birth that rapidly turns yellow or blond. They manifest the development of minimal-to-moderate amounts of cutaneous and ocular pigment. Some patients may have with white hair in the warmer areas (scalp and axilla) and progressively darker hair in the cooler areas (extremities). This variant phenotype is due to a loss of tyrosinase activity above 35-37 degrees C.
  • 配列類似性Belongs to the tyrosinase family.
  • 細胞内局在Melanosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATN antibody
    • CMM8 antibody
    • LB24 AB antibody
    • LB24-AB antibody
    • Monophenol monooxygenase antibody
    • OCA1 antibody
    • OCA1A antibody
    • OCAIA antibody
    • Oculocutaneous albinism IA antibody
    • SHEP3 antibody
    • SK29 AB antibody
    • SK29-AB antibody
    • Tumor rejection antigen AB antibody
    • TYR antibody
    • TYRO_HUMAN antibody
    • tyrosinase (oculocutaneous albinism IA) antibody
    • Tyrosinase antibody
    see all

Anti-Tyrosinase antibody 画像

  • ICC/IF image of ab61294 stained Malme-3M cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61294, 5µg/ml) overnight at +4°C. The secondary antibody (green) was a96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Tyrosinase antibody (ab61294) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

Anti-Tyrosinase antibody (ab61294) 使用論文

This product has been referenced in:
  • Liang H  et al. CtBP2 Downregulation during Neural Crest Specification Induces Expression of Mitf and REST, Resulting in Melanocyte Differentiation and Sympathoadrenal Lineage Suppression. Mol Cell Biol 31:955-70 (2011). WB ; Quail . Read more (PubMed: 21199918) »

See 1 Publication for this product

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