製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to TYK2
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: PVCHLRLLAQ AEGEPCYIRD SGVAPTDPGP ESAAGPPTHE VLVTGTGGIQ WWPVEEEVNK EEGSSGSSGR NPQASLFGKK AKAHKAVGQP ADRPREPLWA , corresponding to amino acids 276-375 of Human TYK2

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab223733 as a replacement.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab57678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 134 kDa.
IHC-P Use a concentration of 3 µg/ml.

ターゲット情報

  • 機能
    Probably involved in intracellular signal transduction by being involved in the initiation of type I IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain.
  • 組織特異性
    Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines.
  • 関連疾患
    Defects in TYK2 are the cause of protein-tyrosine kinase 2 deficiency (TYK2 deficiency) [MIM:611521]; also known as autosomal recessive hyper-IgE syndrome (HIES) with atypical mycobacteriosis. TYK2 deficiency consists of a primary immunodeficiency characterized by recurrent skin abscesses, pneumonia, and highly elevated serum IgE.
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • ドメイン
    The FERM domain mediates interaction with JAKMIP1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • JTK 1 antibody
    • JTK1 antibody
    • Non receptor tyrosine protein kinase 2 antibody
    • Non receptor tyrosine protein kinase TYK2 antibody
    • Non-receptor tyrosine-protein kinase TYK2 antibody
    • OTTHUMP00000232745 antibody
    • OTTHUMP00000232746 antibody
    • OTTHUMP00000232748 antibody
    • Protein Tyrosine Kinase 2 antibody
    • TYK 2 antibody
    • Tyk2 antibody
    • TYK2_HUMAN antibody
    • Tyrosine kinase 2 antibody
    see all

画像



  • Predicted band size : 134 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: TYK2 knockout HAP1 cell lysate (20 µg)
    Lane 3: Raji cell lysate (20 µg)
    Lane 4: MDA-MB-361 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab57678 observed at 135 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab57678 was shown to specifically react with TYK2 when TYK2 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab57678 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)

    secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • TYK2 antibody (ab57678) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymphoma.


  • Predicted band size : 134 kDa
    TYK2 antibody (ab57678) at 1ug/lane + HeLa cell lysate at 25ug/lane.

参考文献

ab57678 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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