Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab223733 as a replacement.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 134 kDa.
Use a concentration of 3 µg/ml.
Probably involved in intracellular signal transduction by being involved in the initiation of type I IFN signaling. Phosphorylates the interferon-alpha/beta receptor alpha chain.
Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines.
Defects in TYK2 are the cause of protein-tyrosine kinase 2 deficiency (TYK2 deficiency) [MIM:611521]; also known as autosomal recessive hyper-IgE syndrome (HIES) with atypical mycobacteriosis. TYK2 deficiency consists of a primary immunodeficiency characterized by recurrent skin abscesses, pneumonia, and highly elevated serum IgE.
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.
The FERM domain mediates interaction with JAKMIP1.
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: TYK2 knockout HAP1 cell lysate (20 µg) Lane 3: Raji cell lysate (20 µg) Lane 4: MDA-MB-361 cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab57678 observed at 135 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab57678 was shown to specifically react with TYK2 when TYK2 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab57678 and ab181602 (loading control to GAPDH) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)
secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
IHC-P - TYK2 antibody (ab57678)
TYK2 antibody (ab57678) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymphoma.
Western blot - TYK2 antibody (ab57678)
Predicted band size : 134 kDa TYK2 antibody (ab57678) at 1ug/lane + HeLa cell lysate at 25ug/lane.