製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TXNRD1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human TXNRD1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Testis and Spleen tissue lysates as well as the following whole cell lysates: HeLa; HepG2; HEK293; MCF7.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 71 kDa). Abcam recommends using milk as the blocking agent (3%).

ターゲット情報

  • 機能
    Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
  • 組織特異性
    Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
  • 配列類似性
    Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
    Contains 1 glutaredoxin domain.
  • ドメイン
    The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.
  • 翻訳後修飾
    The N-terminus of isoform 5 is blocked.
    ISGylated.
  • 細胞内局在
    Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cytoplasmic antibody
    • Gene associated with retinoic and IFN-induced mortality 12 protein antibody
    • Gene associated with retinoic and interferon-induced mortality 12 protein antibody
    • Gene associated with retinoid IFN induced mortality 12 protein antibody
    • GRIM 12 antibody
    • GRIM-12 antibody
    • GRIM12 antibody
    • KDRF antibody
    • KM 102 derived reductase like factor antibody
    • KM-102-derived reductase-like factor antibody
    • MGC9145 antibody
    • Oxidoreductase antibody
    • Thioredoxin reductase 1 antibody
    • Thioredoxin reductase 1 cytoplasmic antibody
    • Thioredoxin reductase GRIM 12 antibody
    • Thioredoxin reductase TR1 antibody
    • TR 1 antibody
    • TR antibody
    • TR1 antibody
    • TRXR 1 antibody
    • TRXR1 antibody
    • TRXR1_HUMAN antibody
    • TXNR antibody
    • TXNRD 1 antibody
    • Txnrd1 antibody
    see all

画像

  • All lanes : Anti-TXNRD1 antibody (ab113904) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human spleen tissue lysate - total protein (ab29699)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,37 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

プロトコール

参考文献

ab113904 has not yet been referenced specifically in any publications.

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