The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Nicotinic acid adenine dinucleotide phosphate (NAADP) receptor that may function as one of the major voltage-gated Ca(2+) channels (VDCC) across the lysosomal membrane. May be involved in smooth muscle contraction.
Widely expressed. Expressed at high level in liver and kidney.
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. Two pore calcium channel subfamily.
Each of the two internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
Lysosome membrane. Only the acidic lysosomal fraction is sensitive to NAADP.
ab111145, at 3.75 µg/ml, staining Two pore calcium channel protein 2 in formalin fixed, paraffin embedded Human colon tissue by Immunohistochemistry. Followed by biotinylated secondary antibody, alkaline phosphatase streptavidin and chromogen.
ab111145, at 3.75 µg/ml, staining Two pore calcium channel protein 2 in formalin fixed, paraffin embedded Human pancreas tissue by Immunohistochemistry. Followed by biotinylated secondary antibody, alkaline phosphatase streptavidin and chromogen.