Anti-TWISTNB 抗体 (ab99305)
Key features and details
- Rabbit polyclonal to TWISTNB
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TWISTNB antibody -
製品の詳細
Rabbit polyclonal to TWISTNB -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide within Human TWISTNB aa 250-350. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_001002926.1
Database link: Q3B726 -
ポジティブ・コントロール
- Whole cell lysate from HeLa and 293T cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99305の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 37 kDa. |
ターゲット情報
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機能
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters. -
組織特異性
Widely expressed. Expressed in all fetal and adult tissues tested, with highest expression in fetal lung, liver, and kidney, and low expression in all adult tissues. -
配列類似性
Belongs to the eukaryotic RPA43 RNA polymerase subunit family. -
細胞内局在
Nucleus, nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 221830 Human
- Omim: 608312 Human
- SwissProt: Q3B726 Human
- Unigene: 353035 Human
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別名
- DNA directed RNA polymerase I subunit RPA43 antibody
- DNA-directed RNA polymerase I subunit RPA43 antibody
- RPA43 antibody
see all
画像
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All lanes : Anti-TWISTNB antibody (ab99305) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg
Lane 2 : Hela whole cell lysate at 15 µg
Lane 3 : Hela whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 10 seconds
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab99305 は 1 報の論文で使用されています。
- Yin X et al. RNA polymerase I subunit 12 plays opposite roles in cell proliferation and migration. Biochem Biophys Res Commun 560:112-118 (2021). PubMed: 33984768