製品の概要

  • 製品名Anti-Twist antibody
    Twist 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Twist
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Chicken, Cow, Cat, Dog, Pig, Chimpanzee, Zebrafish
  • 免疫原

    Synthetic peptide: IQTLKLAARY IDFLYQVLQS DELDSKMASC SYVAHERLSY AFSVWRMEGA , corresponding to amino acids 146-195 of Human Twist

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).Can be blocked with Human Twist peptide (ab111667).

Abcam recommends blocking with BSA.

ターゲット情報

  • 機能Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
  • 組織特異性Subset of mesodermal cells.
  • 関連疾患Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
    Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
    Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACS3 antibody
    • B-HLH DNA binding protein antibody
    • bHLHa38 antibody
    • BPES2 antibody
    • BPES3 antibody
    • Class A basic helix-loop-helix protein 38 antibody
    • CRS1 antibody
    • H-twist antibody
    • OTTHUMP00000116043 antibody
    • SCS antibody
    • TWIST antibody
    • Twist basic helix loop helix transcription factor 1 antibody
    • Twist homolog 1 (Drosophila) antibody
    • Twist homolog 1 antibody
    • TWIST homolog of drosophila antibody
    • Twist related protein 1 antibody
    • Twist-related protein 1 antibody
    • TWIST1 antibody
    • TWST1_HUMAN antibody
    see all

Anti-Twist antibody 画像

  • Anti-Twist antibody (ab49254) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000

    Predicted band size : 21 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • ab49254 staining Twist in mouse lung adencarcinoma induced by urethane tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 4% serum for 2 hours at 25°C followed by incubation with the primary antibody, at a 1/100 dilution, for 16 hours at 4°C. A biotinylated goat anti-rabbit IgG polyclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

  • ab49254 staining Twist in human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citric buffer, pH 6.0 prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/50 and incubated with the sample for 12 hour at 4°C. An HRP-conjugated goat anti-rabbit polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

  • Immunohistochemistry of Uterus tissue at an antibody concentration of 4-8µg/ml using anti-TWIST1 antibody (ab49254)

  • All lanes : Anti-Twist antibody (ab49254) at 2.5

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 21 kDa

Anti-Twist antibody (ab49254) 使用論文

This product has been referenced in:
  • Verrill C  et al. Altered expression of epithelial-to-mesenchymal transition proteins in extraprostatic prostate cancer. Oncotarget 7:1107-19 (2016). Read more (PubMed: 26701730) »
  • Cui Y  et al. Asparaginyl endopeptidase promotes the invasion and metastasis of gastric cancer through modulating epithelial-to-mesenchymal transition and analysis of their phosphorylation signaling pathways. Oncotarget 7:34356-70 (2016). Human . Read more (PubMed: 27102302) »

See all 18 Publications for this product

Product Wall

Thank you for contacting us.

I can confirm that the recommended concentration for this product in Western Blot is 2.5ug/ml. I have also reviewed the publication listed on the datasheet and found that they had used a 2.0ug/ml concentration ...

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Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (3T3)
Gel Running Conditions Reduced Denaturing (12% Bis tris)
Loading amount 50 µg
Specification 3T3
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 30 2015

Application IHC - Wholemount
Sample Zebrafish Embryo (wholemount)
Specification wholemount
Username

Abcam user community

Verified customer

投稿 Dec 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (gel 11%)
Sample Human Cell lysate - whole cell (ovarian cancer cell line SKOV3)
Specification ovarian cancer cell line SKOV3
Blocking step blocking One (Nacalai Tesque) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 27°C
Username

宣 若橋

Verified customer

投稿 Jul 08 2013

Thank you for your updates.

I am very sorry to hear that the customer is not satisfied with the staining.

I can certainly offer either a new vial as a free of charge replacement or a credit note which can be used for future purchase...

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Thank you for your response.

After reading through the detailed protocol you kindly forwarded to Abcam, I would make some comments:

1) Image: Could you please save the image as a jpeg file and send it back to us.

2) Antige...

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Application Western blot
Sample Mouse Cell lysate - whole cell (NMuMG)
Loading amount 75 µg
Specification NMuMG
Gel Running Conditions Reduced Denaturing (12)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 19 2012

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products

I would be pleased to arrange a replacement product free of charge. I have added a vial of ab50887 to your next order.

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Thank you for taking time to provide that information and for contacting us with this problem. I am sorry to hear this antibody hasnotbeen providing satisfactory results.

The details provided will enable us to investigate this case and will ...

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Thank you for contacting me and for keeping me updated about the results with the Tbx1 and Twist antibodies. I'm sorry to hear that the results are not good at this point, but please let me know if there is anything I can do to help in that situation. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"