製品の概要

  • 製品名Anti-Twinkle antibody
    Twinkle 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Twinkle
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 612-661 (GDVGVFPLEF NKNSLTFSIP PKNKARLKKI KDDTGPVAKK PSSGKKGATT) of Human Twinkle (NP_068602)

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 77 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

ターゲット情報

  • 機能Involved in mitochondrial DNA (mtDNA) metabolism. Could function as an adenine nucleotide-dependent DNA helicase. Function inferred to be critical for lifetime maintenance of mtDNA integrity. In vitro, forms in combination with POLG, a processive replication machinery, which can use double-stranded DNA (dsDNA) as template to synthesize single-stranded DNA (ssDNA) molecules. May be a key regulator of mtDNA copy number in mammals.
  • 組織特異性High relative levels in skeletal muscle, testis and pancreas. Lower levels of expression in the heart, brain, placenta, lung, liver, kidney, spleen, thymus, prostate, ovary, small intestine, colon and leukocytes. Expression is coregulated with MRPL43.
  • 関連疾患Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant, 3
    Sensory ataxic neuropathy dysarthria and ophthalmoparesis
    Mitochondrial DNA depletion syndrome 7
  • 配列類似性Contains 1 SF4 helicase domain.
  • 細胞内局在Mitochondrion matrix > mitochondrion nucleoid. Colocalizes with mtDNA in mitochondrial nucleoids, a nucleoproteins complex consisting of a number of copies of proteins associated with mtDNA, probably involved in mtDNA maintenance and expression.
  • Information by UniProt
  • 参照データベース
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Anti-Twinkle antibody 画像

  • Anti-Twinkle antibody (ab83207) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 77 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

Anti-Twinkle antibody (ab83207) 使用論文

ab83207 has not yet been referenced specifically in any publications.

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