製品の概要

  • 製品名Anti-TWEAKR antibody
    TWEAKR 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TWEAKR
  • 特異性This antibody reacts with TWEAKR
  • アプリケーション適用あり: ICC/IF, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human IgG1 Fc-tagged TWEAKR protein

  • ポジティブ・コントロール
    • HepG2 cell lysate, 293 cells and human liver tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 14 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 2.5 µg/ml.

ターゲット情報

  • 機能Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
  • 組織特異性Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
  • 配列類似性Contains 1 TNFR-Cys repeat.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 266 antibody
    • CD266 antibody
    • CD266 antigen antibody
    • FGF inducible 14 antibody
    • FGF-inducible 14 antibody
    • Fibroblast growth factor inducible immediate early response protein 14 antibody
    • Fibroblast growth factor-inducible immediate-early response protein 14 antibody
    • FN 14 antibody
    • FN14 antibody
    • TNFRSF 12A antibody
    • TNFRSF12A antibody
    • TNR12_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 12A antibody
    • TWEAK R antibody
    • Tweak receptor antibody
    • Tweak-receptor antibody
    • TweakR antibody
    see all

Anti-TWEAKR antibody 画像

  • All lanes : Anti-TWEAKR antibody (ab62496) at 1 µg/ml

    Lane 1 : mock transfected 293 cells
    Lane 2 : Fn14-GPI transfected 293 cells

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 14 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of TWEAKR in human liver tissue with ab62496 antibody at 2.5 µg/ml.


  • Predicted band size : 14 kDa
  • ICC/IF image of ab62496 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62496, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunofluorescence of Fn14 in Human Liver cells using ab62496 at 20 ug/ml.

Anti-TWEAKR antibody (ab62496) 使用論文

This product has been referenced in:
  • Affò S  et al. Transcriptome analysis identifies TNF superfamily receptors as potential therapeutic targets in alcoholic hepatitis. Gut : (2012). IHC-P ; Human . Read more (PubMed: 22637703) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"