製品の概要

  • 製品名Anti-TWEAK antibody
    TWEAK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TWEAK
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Horse
  • 免疫原

    Synthetic peptide within (GLLLAVVSLG SRASLSAQEP AQEELVAEED QDPSELNPQT EESQDPAPFL) corresponding to N terminal amino acids 36-85 of human TWEAK (NP_003800).

  • ポジティブ・コントロール
    • 721_B cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines.
  • 組織特異性Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
  • 配列類似性Belongs to the tumor necrosis factor family.
  • 翻訳後修飾The soluble form derives from the membrane form by proteolytic processing.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • APO 3 ligand antibody
    • APO 3L antibody
    • APO3 ligand antibody
    • APO3/DR3 ligand antibody
    • APO3L antibody
    • DR3LG antibody
    • MGC129581 antibody
    • MGC20669 antibody
    • secreted form antibody
    • TNF-related weak inducer of apoptosis antibody
    • TNF12_HUMAN antibody
    • TNFSF 12 antibody
    • Tnfsf12 antibody
    • TNFSF12 protein antibody
    • Tumor necrosis factor (ligand) superfamily member 12 antibody
    • Tumor necrosis factor ligand superfamily member 12 antibody
    • Tumor necrosis factor superfamily member 12 antibody
    • TWEAK antibody
    • UNQ181/PRO207 antibody
    see all

Anti-TWEAK antibody 画像

  • Anti-TWEAK antibody (ab86287) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 27 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

Anti-TWEAK antibody (ab86287) 使用論文

ab86287 has not yet been referenced specifically in any publications.

Product Wall

Thank you for sending along the western blot images. I just have a few additional questions: 1. Are the samples of TWEAK transfected cell extracts or are these samples purified recombinant TWEAK protein or are they untransfected cell lysa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"